Quantitative Analysis of Flesinoxan in Plasma and Urine at the pg/ml Level Using GC-ECD

  • M. P. van Berkel
  • H. de Bree
  • K. Sierat
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 18 A)

Abstract

Ceelan et al. [1] developed an HPLC method for the determination in plasma of flesinoxan (given as the hydrochloride; DU 23973), a relatively large and polar molecule of low volatility, shown on left in the diagram below. However, on the basis of the results of the first safety and tolerance study the dose was lowered to 0.25 mg/person, resulting in plasma levels in the pg/ml range. For the pharmacokinetic monitoring of the drug at this low dose, the HPLC method was unsuitable. A new analytical procedure had to be developed using a completely different strategy aiming at utmost sensitivity. GC-ECD⊗ is a highly sensitive technique which could be suitable after hydrolysis of the drug to the FBz and then esterification with PFBB

Keywords

Methyl Isobutyl Isobutyl Ketone Phonic Acid Pharmacokinetic Monitoring Perchloric Acid Extraction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Ceelen, P.R.J., et al. (1986) in V. 16, this series (Reid, E., et al., eds.), 297.Google Scholar
  2. 2.
    Knapp, D.R. (1979) Handbook of Analytical Devivatization Reactions, Wiley-Interscience, New York, p. 47.Google Scholar

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • M. P. van Berkel
    • 1
  • H. de Bree
    • 1
  • K. Sierat
    • 1
  1. 1.Duphar Research LaboratoriesWeespThe Netherlands

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