Enantiomeric Analysis of 2-Phenylpropionic Acid NSAID’s in Biological Fluids by HPLC

  • A. J. Hutt
  • J. Caldwell
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 18 A)

Abstract

The importance of stereochemistry in the disposition of the 2-⌽-propionic acid NSAID’s* is outlined. General approaches for the Chromatographic resolution of these agents by both direct and indirect methods are briefly reviewed. Problems associated with the choice of HPLC mobile phase for the resolution of, and derivatization methods for the formation of, stable diastereoisomeric derivatives are illustrated with reference to pirprofen. The application of chiral mobile phases and commercially available HPLC CSP’s to the analysis of these agents is examined, and problems which may arise during the ‘work-up’ of samples of biological origin are discussed.

Keywords

Mandelic Acid Tiaprofenic Acid Chromatographic Resolution Amide Derivative Derivatizing Agent 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviation

NSAID

non-steroidal antirinflammatory drug

THF

tetrahydrofuran

DMOA

N,N-dimethyloctylamine

CSP

chiral stationary phase

RP

reversed phase

MS

mass spectrometry (EI, electron impact)

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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • A. J. Hutt
    • 1
  • J. Caldwell
    • 2
  1. 1.Department of PharmacyBrighton PolytechnicBrightonUK
  2. 2.Department of Pharmacology and ToxicologySt. Mary’s Hospital Medical SchoolLondonUK

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