HPLC Analysis of Anti-Inflammatory Agents (Non-Steroidal and Steroidal)

  • J. C. McElnay
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 18 A)

Abstract

AI* agents in biological fluids are commonly analyzed chromatographically, maybe by GC but usually by HPLC — typically RP — with UV detection (sometimes fluorescence or EC). HPLC is also convenient in percutaneous absorption studies on AI agents in gels and creams. Solvent extraction is often not necessary if the drug concentration, e.g. in plasma, is relatively high: precipitation of plasma proteins is often sufficient, provided that a pre-column is used.

Keywords

Analytical Column Mefenamic Acid Typical Chromatogram Percutaneous Absorption Fluocinolone Acetonide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviation

AI

anti-inflammatory

EC

electrochemical

Fa

fluocinolone acetonide

i.s.

internal standard

r

correlation coefficient

RP

reversed phase

SDS

sodium dodecyl sulphate

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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • J. C. McElnay
    • 1
  1. 1.Deparment of PharmacyThe Queen’s University of Belfast, Medical Biology CentreBelfast, N. IrelandUK

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