Scanning Force Microscopy on Living Virus-Infected Cells
Using scanning force microscopy (SFM), it is possible to follow processes on the membrane of single living cells. Monkey kidney cells were imaged under normal growth conditions with a resolution of approximately 10 nm. Upon adding a suspension of pox virus, pronounced changes in the cell membrane were observed. Image sequences of these processes on the cell surface can be monitored over time. The cell membrane was studied to determine how it is affected by changes in temperature. The end of the micropipette holding the cell was also examined by SFM.
KeywordsVaccinia Virus Monkey Kidney Cell Single Living Cell Patch Clamp Pipette Scanning Force Microscopy Image
Unable to display preview. Download preview PDF.
- 11.W. Häberle, J.K. Hörber, F. Ohnesorge, D.P.E. Smith, and G. Binnig, In situ investigation of single living cells infected by viruses, Ultramicroscopy, 42–44: 1161–1167 (1992).Google Scholar
- 13.B. Sakmann and E. Neher, “Single-Channel Recording,” Plenum Press, New York, 37–51 (1983).Google Scholar
- 14.J.K.H. Hörber, W. Häberle, F. Ohnesorge, G. Binnig, H.G. Liebich, C.P. Czerny, H. Mahnel, and A. Mayr, Investigation of living cells in the nanometer regime with the scanning force microscope, Scanning Microscopy 6: 919–930 (1992).Google Scholar
- 15.G.V. Stokes, High-voltage electron microscope study of the release of vaccina virus from whole cells, J. Virology 18: 636–643 (1976).Google Scholar