Potency Assay of Inactivated Influenza and Poliovaccine
Influenza virus naturally infects humans, pigs, horses, seals, and many different wild and domestic avian species (Hinshaw and Webster, 1982). Inactivated vaccines against influenza are used in humans and in horses and experimental vaccines have been studied in swine and domestic poultry. Vaccination in humans has been shown to induce serum antibody to influenza hemagglutinin (HA), which is capable of virus neutralization and prevention of infection (Dowdle et al., 1974; Hobson et al., 1979). It is therefore important to measure vaccine HA antigen concentration in order to standardize the dose required for effective immunization. Several years ago, single-radial diffusion (SRD) techniques were developed for in vitro potency testing of human influenza vaccines (Wood et al., 1977). The SRD test was shown to be technically simple, reproducible, and sensitive and produced vaccine potency data that related to antigenicity of vaccines (Wood et al., 1977).
KeywordsInfluenza Virus Avian Influenza Influenza Vaccine Potency Test Domestic Poultry
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