Current Electron Microscopic Methods in Diagnostic Virology

  • Robert D. O. Devine
Part of the Applied Virology Research book series (AVIR, volume 3)


The development of electron microscopy as applied to diagnostic virology began with the occasional, yet nonetheless urgent, need to differentiate herpes zoster infections from mild or atypical variola. Laboratory methods had been designed to identify these viruses but only electron microscopy enabled the differentiation to be made within minutes of receiving the clinical specimens. A description of the electron microscopic procedures used to successfully identify herpes zoster or variola in vesicular fluid specimens taken from infected patients was published in 1948 by Nagler and Rake.


Herpes Zoster Clinical Specimen Immunoelectron Microscopy Vesicle Fluid Molluscum Contagiosum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Anderson, N., Doane, F.W. (1972). Appl. Microbiol. 24, 95–496.Google Scholar
  2. Anderson, N., Doane, F.W. (1973). Can.J. Microbiol. 19, 585–589.PubMedCrossRefGoogle Scholar
  3. Appleton, H (1987). CIBA Found. Symp. 128, 108–25.PubMedGoogle Scholar
  4. Appleton, H., Buckley, M., Thom, B.T. Cotton, J.L. and Henderson, S. (1977). Lancet 1, 409.PubMedCrossRefGoogle Scholar
  5. Bishop, R.F., Davidson, G.P., Holmes, H. (1973). Lancet 2, 1497.Google Scholar
  6. Brenner, S. and Horne, R.W. (1959). Biochim. Biophys. Acta. 34,103–110. Caul, E.O., Paver, W.K. and Clark, S.K.R.(1975). Lancet 1, 1192.Google Scholar
  7. Caul, E.O., Ashley, C.R. and Egglestone, S.I. (1977). Med. Lab. Sci. 34, 259–263.PubMedGoogle Scholar
  8. Cubitt WD (1987). CIBA Found. Symp. 128, 26–43.Google Scholar
  9. Cubitt WD (1990). Clin. Gastroenterol. 4, 643–56.Google Scholar
  10. Fang, Z.Y., Ye, Q. HD, M.S., Dong, H., Qing, S., Penaranda, M.E., Wen, I., Glass, R.T., (1989). J. Infect. Dis. 160, 948–953.PubMedCrossRefGoogle Scholar
  11. Hewett, T.H., Bryden, A.S. and Davies, H. (1973). Lancet Z, 1497.Google Scholar
  12. Hewett, T.H., Beards, G.M., Brown,D.W., Sanders, R.C. (1987). CIBA Found. Symp. 128, 238–249.Google Scholar
  13. Gary, G.W., JNR., Hierholzer, J.C. and Black, R.E. (1979). J.Clin. Microbiol. 10, 96.PubMedGoogle Scholar
  14. Gordon, S.M., Oshiro, L.S., Jarvis. W.R., Donenfeld, D., Ho, M.S., Taylor, F., Greenberg, H.B., Glass, R., Madore, H.P., Dolin, R., (1990). Am. J.Epidemiol. 131, 702–710.PubMedGoogle Scholar
  15. Hammond, G.W., Hazelton, P.R., Chuang, I., Klisko, B. (1981). J. Clin. Microbiol. 14, 210–221.PubMedGoogle Scholar
  16. Hayashi, Y., Ando, T., Utagawa, E., Sekine, S., Okada, S., Yabuuchi, K., Miki, T., Ohash, M. (1989). J. Clin. Microbiol. 27, 1728–1733.PubMedGoogle Scholar
  17. Herrmann., J.E.,(1984). Lancet, 2, 1405.Google Scholar
  18. Herrmann, J.E., Nowak, N.A., Perron-Henry, D.M., Hudson, R.W., Cubitt, W.D., Blacklow, N.R. (1990). J. Infect. Dis. 161, 226–229.PubMedCrossRefGoogle Scholar
  19. Hopley, J.F.A., Doane, F.W. (1985). J. Virol. Meth. 12, 135–147.CrossRefGoogle Scholar
  20. Home, R.W., (1965). In Techniques for Electron Microscopy (R. Bullock and P. Petrusz, eds.) pp. 328–355. Blackwell Scientific, Oxford.Google Scholar
  21. Kapikian, A.Z., Wyatt, R.G., Dolin, R., Thornhill, T.S., Kalica, A.R., and Chanock, R.M., (1972). J. Virol. 10, 1075.Google Scholar
  22. Katz, D., Straussman, Y., Shahar, A., Kohn, A., (1980). J. Immunol. Meth. 38, 171–174.CrossRefGoogle Scholar
  23. Kjeldsberg, E. (1986). J. Virol. Meth. 14, 321–333.CrossRefGoogle Scholar
  24. Kjeldsberg, E., Anestad, G., Greenberg, H., Orstavik, I., Pedersen, R., Sletteb,E. (1989). Scand. J. Infect. Dis. 21, 521–526.PubMedCrossRefGoogle Scholar
  25. Konno, T., Suzuki, H., Ishida, N., Chiba, R., Mochizuki, K., Tsunoda, A., (1982). J. Med. Virol. 9, 11–17.PubMedCrossRefGoogle Scholar
  26. Lee, T.W., Kurtz, J.B., (1981). J. Gen. Virol. 57, 421–424.PubMedCrossRefGoogle Scholar
  27. Leers, W.D., Kasupski, G., Fralick, R., Wartman, S., Garcia, J., Gary, W., (1987). Am J. Public Health. 77, 291–295.PubMedCrossRefGoogle Scholar
  28. Lew, J.F., Moe, C.L., Munroe, S.S., Allen, J.R., Harrison, B.M., Forrester, B.D., Stine, S.E., Woods, P.A., Hierholzer, J.C., Herrmann, J.E., Blacklow, N.R., Bartlett, A.V., Glass, R.I., (1991). J, Infect. Dis. 164, 673–678.Google Scholar
  29. Lewis, D.C., Lightfoot, N.F., Pether, J.V., (1988). J. Clin. Microbiol. 26, 938–942.PubMedGoogle Scholar
  30. Lewis, D.C., (1990). J. Med. Virol. 30, 77–81.CrossRefGoogle Scholar
  31. Madely, C.R. and Cosgrove, P.B. (1976). Lancet 1, 199.CrossRefGoogle Scholar
  32. Madore, H.P., Treanor, J.J., Buja, R., Dolin, R (1990). J. Med. Virol. 32, 96–101.PubMedCrossRefGoogle Scholar
  33. Monroe, S.S., Glass, R.I., Noah, N., Hewett, T.H., Caul, E.O., Ashton, C.I., Curry, A., Field, A.M., Madely, R., Pead, P.J. (1991). J. Med. Virol. 33, 193–198.PubMedCrossRefGoogle Scholar
  34. Madore, H.P., Treanor, J.J., Buja, R., Dolin, R (1990). J. Med. Virol. 32, 96–101.PubMedCrossRefGoogle Scholar
  35. Nagler, F.P.O. and Rake, G (1948). J. Bacteriol. 55, 45–51.PubMedGoogle Scholar
  36. Okada, S., Sekine, S., Ando, T., Hayashi, Y., Murao, M., Yabuuchi, K., Miki, T., Ohashi, M., (1990). J. Clin. Microbiol. 28, 1244–1248.PubMedGoogle Scholar
  37. Polak, J.M., Varndell, I.M. eds. (1984). Immunolabelling for Electron Microscopy. Elsevier, New York.Google Scholar
  38. Roth, J., (1983). In Techniques in Immunochemistry Vol. 2, 215. ??????Google Scholar
  39. Sekla, L., Stackiw, W., Dzogan, S., Sargeant, D. (1989). Can. Med. Assoc. J.149,1461–1464.Google Scholar
  40. Storr, J., Rice, S., Phillips, A.D., Price, E., Walker-Smith, J.A., (1986). J. Pediatr. GastroenteroI. Nutr. 5, 576–580.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Robert D. O. Devine
    • 1
  1. 1.Provincial Laboratory of Public HealthUniversity of AlbertaEdmontonCanada

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