Basement Membrane Biosynthesis as a Biochemical Index of Angiogenesis in Chick Chorioallantoic Membrane

  • Nikos E. Tsopanoglou
  • Michael E. Maragoudakis
Chapter
Part of the NATO ASI Series book series (NSSA, volume 298)

Abstract

The chorioallantoic membrane (CAM) of the chick is formed by fusion of the somatic mesoderm of the chorion with the splachnic mesoderm of the allantois during the 4th to 5th day of embryonic development. After 6 days of incubation, the CAM is covering a surface area of approximately 6 cm2. By day 10, and extending to day 14, mean surface area of the CAM is approximately 65 cm2. This expansion of CAM is accompanied by an increased complexity of the patterns of capillary microvessels. The number of capillary vessels per square centimetre is increased about 150% from day 7 to day 12 and the intercapillary distances have been reduced about 40% during the same period (1). This highly vascularized membrane serves as the first respiratory system of the avian.

Keywords

Vascular Density Collagenous Protein Chorioallantoic Membrane Cortisone Acetate Chick Chorioallantoic Membrane 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Defouw, O.D., Rizzo, V.J., Steinfeld, R. and Feinberg, R.N. (1989). Mapping of the microcirculation in the chick chorioallantoic membrane during normal angiogenesis. Microvasc. Res., 38: 136–147.PubMedCrossRefGoogle Scholar
  2. 2.
    D’Amore, P.A. and Thompson, R.N. (1987). Mechanism of angiogenesis. Ann. Rev. Physiol., 49: 453–464.CrossRefGoogle Scholar
  3. 3.
    Maragoudakis, M.E., Panoutsakopoulou, M. and Sarmonica, M. (1988). Rate of basement membrane biosynthesis as an index of angiogenesis. Tissue and Cell, 20: 531–539.PubMedCrossRefGoogle Scholar
  4. 4.
    Maragoudakis, M.E., Missirlis, E., Sarmonika, M., Panoutsakopoulou, M. and Karakioulakis, G. (1989). Basement membrane biosynthesis as a target to tumour therapy. J. Pharmacol. Exp. Ther., 252: 753–757.Google Scholar
  5. 5.
    Karakioulakis, G., Missirlis, E., Aletras, A. and Maragoudakis, M.E. (1988). Degradation of intact basement membranes by human and murine tumour enzymes. Biochim. Biophys. Acta. 967: 163–175.CrossRefGoogle Scholar
  6. 6.
    Harris-Hooker, S.A., Gajdusek, C.M, Wight, T.N. and Schwartz, S.M. (1983). Neovascular responses induced by cultured aortic endothelial cells. J. Cell. Physiol., 114: 302–310.PubMedCrossRefGoogle Scholar
  7. 7.
    Scroggs, A.W., Proia, A.D., Smith, C.F., Halperin, E.C. and Klintworth, G.K. (1991). The effect of total body irradiation on cornea neovascularization in the fi scher 344 rat after chemical cauterisation. Invest. Ophthalmol. Visual. Sci., 32: 2105–2111.Google Scholar
  8. 8.
    Kowalski, J., Khwan, H.H., Prionas, S.D., Allison, A.C. and Fajardo, L.F. (1992). Characterisation and application of the disc angiogenesis system. Exp. Mol., 56: 1–19.CrossRefGoogle Scholar
  9. 9.
    Strick, D.M., Waycarter, R.L., Montani, J.P., Gay, W.J. and Adair, T.H. (1991). Morphometric measurements on chorioallantoic membrane vascularity: Effects of hypoxia and hyperoxia. Am. J. Physiol., 260:H1385–H1389.Google Scholar
  10. 10.
    Maragoudakis, M.E., Haralabopoulos, G.C., Tsopanoglou, N.E. and Pipili-Synetos, E. (1995). Validation of collagenous protein synthesis as an index for angiogenesis with the use of morphological methods. Microvasc. Res., 50:215–222.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Nikos E. Tsopanoglou
    • 1
  • Michael E. Maragoudakis
    • 1
  1. 1.Department of Pharmacology, Medical SchoolUniversity of PatrasPatrasGreece

Personalised recommendations