Anti-Neutrophil Cytoplasm Antibodies (ANCA) Increase Neutrophil Adhesion to Cultured Human Endothelium

  • M. T. Keogan
  • I. Rifkin
  • N. Ronda
  • C. M. Lockwood
  • D. L. Brown
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 336)

Summary

ANCA have been demonstrated to activate normal neutrophils in vitro. This study demonstrates that ANCA positive IgG and Fab2 fragments increase the adhesion of normal neutrophils to cultured human umbilical vein endothelium (HUVEC), and that preincubation of the endothelial monolayers with tumour necrosis factor (TNF) to activate the endothelium enhances this effect. Preincubation of neutrophils with antiCD18 monoclonal antibodies inhibited adhesion, suggesting that the observed effect was integrin mediated.

Keywords

Fab2 Fragment Neutrophil Adhesion Normal Neutrophil Recombinant Human Tumour Necrosis Factor Human Umbilical Vein Endothelium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Falk, R.J., Terrell, R.S., Charles, L.A., and Jennette, J.C., 1990, Anti-neutrophil cytoplasmic autoantibodies induce neutrophils to degranulate and produce oxygen radicals in vitro. Proc Natl Acad Sci USA 87: 4115–4119.PubMedCrossRefGoogle Scholar
  2. 2.
    Keogan, M.T., Esnault, V.L.M., Green, A.J., Lockwood, C.M., and Brown, D.L., 1992, Activation of normal neutrophils by antineutrophil cytoplasm antibodies. Clin. Exp.1mmunol. 90: 228–234.CrossRefGoogle Scholar
  3. 3.
    Ewert, B.H., Jennette, J.C.,and Falk, R.J., 1992, Anti-myeloperoxidase antibodies stimulate neutrophils to damage human endothelial cells. Kidneylnt 41: 375–383.Google Scholar
  4. 4.
    Weiss, S.J., 1987, Tissue destruction by neutrophils. N. Engl. J Med. 320: 365–375.Google Scholar
  5. 5.
    Springer, T.A., 1990, Adhesion receptors of the immune system. Nature 346`425–433.PubMedCrossRefGoogle Scholar
  6. 6.
    Jaffe, E.A., Nachman, R.L., Becker, C.G. and Minick, C.R., 1973, Culture of human endothelial cells derived from human umbilical veins: identification by morphological and immunological criteria. J. clin. Invest. 52: 2745–2756:PubMedCrossRefGoogle Scholar
  7. 7.
    Smith, C.W., Marlin, S.D., Rothlein, R., Toman, C. and Anderson, D.C. 1989, Cooperative interactions of LFA-1 and Mac-1 with intercellular adhesion molecule-1 in facilitating adherance and transendothelial migration of human neutrophils in vitro. J clin. Invest. 83: 2008–2012.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • M. T. Keogan
    • 1
  • I. Rifkin
    • 2
  • N. Ronda
    • 2
  • C. M. Lockwood
    • 2
  • D. L. Brown
    • 1
  1. 1.Dept. of Clinical ImmunologyAddenbrooke’s HospitalCambridgeUK
  2. 2.Dept. of Clinical MedicineUniversity of CambridgeCambridgeUK

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