Amyloidogenic Fragments of Amyloid Precursor Protein in Cells Cultured under Leupeptin
The extracellular deposition of β/A4 protein is the most characteristic neuropathological change observed in the brain of patients suffering from Alzheimer’s disease. The accumulation of β/A4 protein is notable at an early stage of the disease, and increases to a high extent during the progression of the disease which, in turn, is thought to be responsible for generation of neurofibrillary tangles leading to neuronal cell death. The β/A4 protein is a proteolytically cleaved product of receptor-like integral transmembrane protein, the amyloid precursor protein (APP).1,2,3 The mechanism and subcellular compartment of β/A4 protein production are still unknown.
KeywordsAmyloid Precursor Protein Microsomal Fraction Mitochondrial Fraction Amyloid Precursor Protein Processing Sucrose Density Gradient Centrifugation
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