Arabidopsis Thaliana and Heterodera Schachtii: A Versatile Model to Characterize the Interaction Between Host Plants and Cyst Nematodes

  • Florian M. W. Grundler
  • Annette Böckenhoff
  • Kay-Peter Schmidt
  • Miroslaw Sobczak
  • Wladislaw Golinowski
  • Urs Wyss
Part of the NATO ASI Series book series (NSSA, volume 268)

Abstract

Arabidopsis thaliana was shown to be a good host plant for a number of plant parasitic nematodes by Sijmons et al. (1992). Several specific properties make this plant an ideal target to study and analyze the interaction with cyst and root-knot nematodes (Wyss and Grundler, 1992a; Sijmons et al., 1994). The thin and translucent roots provide excellent possibilities to observe both, nematode behavior and plant responses by in vivo microscopy (Wyss and Grundler, 1992b; Wyss et al., 1992). In consequence, exactly timed samples for fixation and subsequent ultrastructural examination can be taken (Golinowski and Grundler, 1992; Grundler et al., 1994). The syncytia induced by female juveniles of Heterodera schachtii are usually covered by only a few cell layers. They are therefore amenable to manipulations with microcapillaries. Substances can be injected into the syncytial feeding cells with the aid of specially adapted microinjection equipment to study physiological and biochemical features associated with nematode nutrient uptake (Böckenhoff and Grundler, 1994). In addition a great number of H. schachtii individuals and syncytia can be obtained to prepare extracts for a biochemical and molecular analysis and also for the production of antibodies (Grundler et. al, 1993). Transgenic A. thaliana plants, carrying a GUS reporter gene, enable the observation of plant gene regulation in nematode feeding sites (Goddijn et al., 1993). The application of several complementary approaches may finally elucidate the complex chain of events in the interactions between the plant and H. schachtii.

Keywords

Plant Parasitic Nematode Vascular Cylinder Xylem Element Syncytial Cell Procambial Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Betka, M., Grundler, F. and Wyss, U., 1991, Influence of changes in the nurse cell system (syncytium) on the development of the cyst nematode Heterodera schachtii: Single amino acids, Phytopathology 81:75.CrossRefGoogle Scholar
  2. Böckenhoff, A. and Grundler, F.M.W., 1994, Studies on the nutrient uptake by the beet cyst nematode Heterodera schachtii by in situ microinjection of fluorescent probes into the feeding structures in Arabidopsis thaliana, Parasitology, in press.Google Scholar
  3. Fisher, O.G., 1988, Movement of lucifer yellow in leaves of Coleus blumei Benth., Plant, Cell and Environment, 11:639.CrossRefGoogle Scholar
  4. Goddijn, O. J. M., Lindsey, K., Van der Lee F. M., Klap, J. C. and Sijmons, P. C., 1993, Differential gene expression in nematode induced feeding structures of transgenic harbouring promoter-gusA fusion constructs. Plant J., 4:863.PubMedCrossRefGoogle Scholar
  5. Golinowski, W. and Grundler, F.M.W., 1992, The structure of feeding sites (syncytia) of Heterodera schachtii in roots, stems and leaves of Arabidopsis thaliana, Nematologica 38:413.Google Scholar
  6. Grundler, F., Betka, M. and Wyss, U., 1991, Influence of changes in the nurse cell system (syncytium) on sex determination and development of the cyst nematode Heterodera schachtii: Total amounts of proteins and amino acids, Phytopathology 81:70.CrossRefGoogle Scholar
  7. Grundler, F.M.W., Böckenhoff, A., Golinowski, W., Münch, A., Schmidt, K.-P., Sobczak, M., Davies, K., Burrows, P. and Wyss, U., 1993, Approaches to the analysis of the host-parasite interaction between A. thaliana and gall- and cyst- forming plant parasitic nematodes, in: “Abstracts of the International Conference in Arabidopsis Research”, R. Hangarter, Scholl, R., Davies, K., Feldmann, K., eds., The Ohio State University, Columbus.Google Scholar
  8. Grundler, F.M.W., Golinowski, W. and Wyss, U., 1994, Sedentary endoparasitic nematodes, in: “Arabidopsis, an Atlas of Morphology and Development”, J. Bowman, ed., Springer, New York.Google Scholar
  9. Kammerloher, W. and Schäffner, A. R., 1993, PIP — an A. thaliana specific plasma membrane MIP homologue cloned by expression in mammalian cells, in: “Abstracts of the International Conference in Arabidopsis Research”, R. Hangarter, Scholl, R., Davies, K., Feldmann, K., eds., The Ohio State University, Columbus.Google Scholar
  10. Krauthausen, J. and Wyss, U., 1982, Influence of the cyst nematode Heterodera schachtii on relative changes in the pattern of free amino acids at feeding sites, Physiol. Pl. Path. 21:425CrossRefGoogle Scholar
  11. Sijmons, P.C., Grundler, F.M.W., von Mende, N., Burrows, P.R., and Wyss, U., 1992, Arabidopsis thaliana as a new model host for plant-parasitic nematodes, Plant J. 1:245.CrossRefGoogle Scholar
  12. Sijmons, P.C., von Mende, N. and Grundler, F.M.W., 1994, Plant parasitic nematodes, in: “Arabidopsis”, C. Somerville and Meyerowitz, E., eds., Cold Spring Harbour, New York.Google Scholar
  13. Wyss, U., 1992, Observations on the feeding behaviour of Heterodera schachtii throughout development, including events during moulting, Fundam. appl. Nématol. 15:75.Google Scholar
  14. Wyss, U. and Grundler, F.M.W., 1992a, Seminar: Heterodera schachtii and Arabidopsis thaliana, a model host-parasite interaction. Nematologica 38:488.CrossRefGoogle Scholar
  15. Wyss, U. and Grundler, F.M.W., 1992b, Feeding behaviour of sedentary plant parasitic nematodes, Neth. J. Pl. Pathol. 98: Supplement 2:165.CrossRefGoogle Scholar
  16. Wyss, U., Grundler, F.M.W. and Münch, A., 1992, The parasitic behaviour of second stage juveniles of Meloidogyne incognita in roots of Arabidopsis thaliana, Nematologica 38:98.CrossRefGoogle Scholar
  17. Wyss, U. and Zunke, U., 1986, Observations on the behaviour of second stage juveniles of Heterodera schachtii inside host roots, Rev. Nématol. 9:153.Google Scholar

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Florian M. W. Grundler
    • 1
  • Annette Böckenhoff
    • 1
  • Kay-Peter Schmidt
    • 1
  • Miroslaw Sobczak
    • 1
  • Wladislaw Golinowski
    • 2
  • Urs Wyss
    • 1
  1. 1.Institut für PhytopathologieUniversität KielKielGermany
  2. 2.Katedra BotanikiSGGW WarszawaWarsawPoland

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