Perspectives for Genetically Engineered Antibodies for the Identification of Nematodes
Over the past decade monoclonal antibody (MA) based immunoassays have been developed for routine identification of plant-parasitic nematode species. The information obtained from such immunoassays can be used for advisory systems with regard to crop rotation, pesticide application and certification. Initially, the development of immunoassays was hindered by difficulties with standardization and raising of specific antibodies. Standardization was mainly hampered by the complexity of the samples which usually contain soil and by difficulties with the homogenization of nematodes. Obtaining specific antibodies was impeded by the great similarities in protein composition of closely related nematode species.
KeywordsLight Chain Antibody Fragment Phage Display Library Affinity Maturation Antibody Phage
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