Inhibition of Azoxymethane Initiated Colon Tumor and Aberrant Crypt Foci Development by Bovine Lactoferrin Administration in F344 Rats

  • Hiroyuki Tsuda
  • Kazunori Sekine
  • Joe Nakamura
  • Yoshihiko Ushida
  • Tetsuya Kuhara
  • Nobuo Takasuka
  • Dae Joong Kim
  • Makoto Asamoto
  • Hiroyasu Baba-Toriyama
  • Malcolm A. Moore
  • Hoyoku Nishino
  • Tadao Kakizoe
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 443)

Summary

The influence of bovine lactoferrin (bLf) on colon carcinogenesis was investigated in male F344 rats treated with azoxymethane (AOM). In experiment I, 2% and 0.2% bLf, and Bifidobacterium longum (B. longum) as a positive control at 3% were given in the diet for 4 weeks, along with two s.c. 15 mg/kg injections of AOM on days 1 and 8. The numbers of aberrant crypt foci (ACF) were decreased by both treatments. Similar results were obtained in experiment II of 13 weeks duration. In experiment III, animals were given three weekly injections of AOM and then received 2 or 0.2% bLf, 2% bLf-hydrolysate, or 0.1% bovine lactoferricin (bLfcin) for 36 weeks. No effects indicative of toxicity were noted, but significant reduction in both the incidence and number of adenocarcinomas of the large intestine was observed with almost all the treatments. Thus, the incidences of colon adenocarcinomas in the groups receiving 2 or 0.2% bLf, 2% bLf-hydrolysate, or 0.1% bLfcin were 15%, 25%, 26.3% and only 10%, respectively, in contrast to the 57.5% control value (p < 0.01). ACF values also exhibited reduced development. Investigation of beta-glucuronidase revealed decrease in the cecal contents of animals receiving bLf. In addition, demonstration of enhancement of NK activity by bLf indicated that its inhibitory effects could have been related to elevated immune cytotoxicity.

Keywords

Whey Protein Basal Diet Aberrant Crypt Focus Colon Carcinogenesis Cecal Content 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Hiroyuki Tsuda
    • 1
  • Kazunori Sekine
    • 1
  • Joe Nakamura
    • 1
  • Yoshihiko Ushida
    • 1
  • Tetsuya Kuhara
    • 1
  • Nobuo Takasuka
    • 1
  • Dae Joong Kim
    • 1
  • Makoto Asamoto
    • 1
  • Hiroyasu Baba-Toriyama
    • 1
  • Malcolm A. Moore
    • 1
  • Hoyoku Nishino
    • 2
  • Tadao Kakizoe
    • 3
  1. 1.Chemotherapy DivisionNational Cancer Center Research InstituteChuo-ku, Tokyo 104Japan
  2. 2.Department of BiochemistryKyoto Prefectural University of MedicineKamigyo-ku, Kyoto 602Japan
  3. 3.National Cancer Center Central HospitalChuo-ku, Tokyo 104Japan

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