Evidence for the Existence of a Surface Receptor(s) for Ferriclactoferrin and Ferrictransferrin Associated with the Plasma Membrane of the Protozoan Parasite Leishmania donovani

  • Bradley E. Britigan
  • Troy S. Lewis
  • Michael L. McCormick
  • Mary E. Wilson
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 443)

Summary

Previous work has demonstrated the ability of the promastigote form of the protozoan parasite Leishmania chagasi to utilize iron chelated to lactoferrin and transferrin for growth and metabolism. We have obtained evidence suggesting that the promastigote form of the parasite possesses specific binding sites for lactoferrin and transferrin. Lactoferrin binding appears to be: 1) independent of whether or not the protein contains iron; 2) not inhibited by transferrin: and 3) independent of whether the organism is in log or stationary phase of growth. Transferrin binding is: 1) markedly greater if the protein is iron loaded; 2) inhibited by the presence of lactoferrin; and 3) independent of whether the organism is in log or stationary growth phase. Preliminary ligand blot analyses are consistent with the presence of a protein or proteins which bind lactoferrin and/or transferrin. The relationship to these binding sites to those described in other protozoan species requires further investigation.

Keywords

Visceral Leishmaniasis Protozoan Parasite Trypanosoma Brucei Amastigote Form Promastigote Form 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Bradley E. Britigan
    • 1
    • 2
  • Troy S. Lewis
    • 1
  • Michael L. McCormick
    • 1
    • 2
  • Mary E. Wilson
    • 1
    • 2
    • 3
    • 4
  1. 1.Research ServiceVA Medical Center-Iowa CityIowa CityUSA
  2. 2.Department of Internal MedicineUniversity of Iowa, College of MedicineIowa CityUSA
  3. 3.Department of MicrobiologyUniversity of Iowa, College of MedicineIowa CityUSA
  4. 4.Program in ImmunologyUniversity of Iowa, College of MedicineIowa CityUSA

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