Bovine in Vitro Fertilization

  • J. J. Parrish
  • N. L. First

Abstract

In vitro fertilization in cattle was first reported by Bracket et al. (1982) using surgically recovered oocytes that were matured in vivo and surgically transferred as zygotes to the oviducts of cows. The procedure was later simplified by laparoscopic removal of in vivo-matured oocytes and development of the embryos in oviducts of rabbits (Sirard and Lambert, 1985; Sirard et al., 1985). Although successful, these experiments were not highly efficient in sperm capacitation and required in vivo oocyte maturation, surgery, and development of the embryos in oviducts. Recently systems for producing and culturing bovine embryos totally in vitro have been developed. As a result, bovine embryos are now produced in large numbers for research purposes or in some cases for commercial embryo transfer (Leibfried-Rutledge et al., 1989; Gordon and Lu, 1990). The efficient production of embryos in vitro has required an understanding of the mechanisms of sperm capacitation, maturation of oocytes, and sperm-egg interaction in the fertilization process. Understanding these processes for bovine gametes is the focus of this review.

Keywords

Follicular Fluid Seminal Plasma Oocyte Maturation Zona Pellucida Cumulus Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Aktas, H., Leibfried-Rutledge, M. L., Wheeler, M. B., Rosenkrans, C. F., Jr., and First, N. L., 1990, Maintenance of meiotic arrest in bovine oocytes, Biol. Reprod. 42 (Suppl. 1): 91.Google Scholar
  2. Aoyagi, Y., Fukii, K., Iwazumi, Y., Furudate, M., Fukui, Y., and Ono, H., 1988, Effects of two treatments on semen from different bulls on in vitro fertilization results of bovine oocytes, Theriogenology 30: 973–985.PubMedCrossRefGoogle Scholar
  3. Ball, G. D., Ax, R. L., and First, N. L., 1980, Mucopolysaccharide synthesis accompanies expression of bovine cumulus—oocyte complexes in vitro, in: Functional Correlates of Hormone Receptors in Reproduction ( V. B. Mahesha, T G. Muldoon, B. B. Saxena, and W. A. Sadler, eds.), Elsevier/North-Holland, New York, pp. 561–563.Google Scholar
  4. Ball, G. D., Bellin, M. E., Ax, R. L., and First, N. L., 1982, Glycosaminoglycans in bovine cumulus oocyte complexes: Morphology and chemistry, Mol. Cell. Endocrinol. 28: 113–122.PubMedCrossRefGoogle Scholar
  5. Ball, G. D., Leibfried, M. L., Lenz, R. W, Ax, R. L., Bavister, B. D., and First, N. L., 1983, Factors affecting successful in vitro fertilization of bovine follicular oocytes, Biol. Reprod. 28: 717–725.PubMedCrossRefGoogle Scholar
  6. Bousquet, D., and Brackett, B. G., 1982, Penetration of zona-free hamster ova as a test to assess fertilizing ability of bull sperm after frozen storage, Theriogenology 17: 199–213.PubMedCrossRefGoogle Scholar
  7. Brackett, B. G., Bousquet, D., Boice, M. L., Donawich, W. J., Evans, J. F., and Dressel, M. A., 1982, Normal development following in vitro fertilization in the cow, Biol. Reprod. 27: 147–158.PubMedCrossRefGoogle Scholar
  8. Buccione, R. B. C., Caron, P. J., and Eppig, J. J., 1990, FSH-induced expansion of the mouse cumulus oophorus in vitro is dependent upon a specific factor(s) secreted by the oocyte, Dev. Biol. 138: 16–25.PubMedCrossRefGoogle Scholar
  9. Carlson, D., Black, D. L., and Howe, G. R., 1970, Oviduct secretion in the cow, J. Reprod. Fertil. 22: 549–552.PubMedCrossRefGoogle Scholar
  10. Casu, B., 1985, Structure and biological activity of heparin, Adv. Carbohyd. Chem. Biochem. 43: 51–134.CrossRefGoogle Scholar
  11. Crister, E. S., Leibfried-Rutledge, M. L., Eyestone, W. E., Northey, D. L., and First, N. L., 1986, Acquisition of developmental competence during maturation in vitro, Theriogenology 25: 150.CrossRefGoogle Scholar
  12. Davis, B. K., 1981. Timing of fertilization in mammals: Sperm cholesterol phospholipid ratio as a determinant of the capacitation interval, Proc. Natl. Acad. Sci. U.S.A. 78: 7560–7564.PubMedCrossRefGoogle Scholar
  13. Ehrenwald, E., Parks, J. E., and Foote, R. H., 1988a, Cholesterol efflux from bovine sperm. I. Induction of the acrosome reaction with lysophosphatidylcholine after reducing sperm cholesterol, Gamete Res. 20: 145–157.PubMedCrossRefGoogle Scholar
  14. Ehrenwald, E., Parks, J. E., and Foote, R. H., 1988b, Cholesterol efflux from bovine sperm: II. Effect of reducing sperm cholesterol on penetration of zona-free hamster and in vitro matured bovine ova, Gamete Res. 20: 413–420.PubMedCrossRefGoogle Scholar
  15. Ehrenwald, E., Foote, R. H., and Parks, J. E., 1990, Cholesterol efflux from bovine sperm: Bovine oviductal fluid components and their potential role in sperm cholesterol efflux, Exp. Biol. Med. 25: 195–204.Google Scholar
  16. Eppig, J. J., 1980, Role of serum FSH stimulated cumulus expansion by mouse oocyte—cumulus cell complexes in vitro, Biol. Reprod. 23: 629–633.CrossRefGoogle Scholar
  17. Fayrer-Hosken, R. A., Brackett, B. G., and Brown, J., 1987, Reversible inhibition of rabbit sperm-fertilizing ability by cholesterol sulfate, Biol. Reprod. 36: 878–883.PubMedCrossRefGoogle Scholar
  18. First, N. L., and Parrish, J. J., 1987, In vitro fertilization of ruminants, J. Reprod. Fertil. 34 (Suppl.): 151–165.Google Scholar
  19. First, N. L., Leibfried-Rutledge, M. L., Sirard, M. A., 1988, Cytoplasmic control of oocyte maturation and species differences in the development of maturational competence, in: Meiotic Inhibition: Molecular Control, of Meiosis, (F. P. Haseltine, N. L. First, P. Patinelli, and C. O. Liss, eds.), Alan R. Liss, New York, pp. l-46.Google Scholar
  20. Florman, H. M., and First, N. L., 1988a, The regulation of acrosomal exocytosis. I. Sperm capacitation is required for the induction of acrosome reactions by the bovine zona pellucida in vitro, Dev. Biol. 128: 453–463.PubMedCrossRefGoogle Scholar
  21. Florman, H. M., and First, N. L., 19886, The regulation of acrosomal exocytosis. II. The zona pellucid-inducing acrosome reaction of bovine spermatozoa is controlled by extrinsic positive regulatory elements, Dev. Biol. 128: 464–473.Google Scholar
  22. Florman, H. M., Tombes, R. M., First, N. L., and Babcock, D. F., 1989, An adhesion-associated agonist from the zona pellucida activates G protein-promoted elevations of internal Ca2+ and pH that mediate mammalian sperm acrosomal exocytosis, Dev. Biol. 135: 133–146.PubMedCrossRefGoogle Scholar
  23. Foote, W. D., and Thibault, C., 1969, Recherches experimentales sur la maturation in vitro des ovocytes de truie et de veau, Ann. Biol. Anim. Biochem. Biophys. 9: 327–349.CrossRefGoogle Scholar
  24. Fraser, L. R., and Ahuja, K. K., 1988, Metabolic and surface events in fertilization, Gamete Res. 20: 491–519.PubMedCrossRefGoogle Scholar
  25. Fukuda, A., Ichikawa, M., Naito, K., and Toyoda, Y., 1990, Birth of normal calves resulting from bovine oocytes matured, fertilized, and cultured with cumulus cells in vitro up to the blastocyst stage, Biol. Reprod. 42: 114–119.PubMedCrossRefGoogle Scholar
  26. Go, K. J., and Wolf, D. P, 1985, Albumin-mediated changes in sperm sterol content during capacitation, Biol. Reprod. 32: 145–153.PubMedCrossRefGoogle Scholar
  27. Gordon, I., and Lu, K. H., 1990, Production of embryos in vitro and its impact on livestock production, Theriogenology 33: 77–88.CrossRefGoogle Scholar
  28. Hanada, A., 1986, In vitro fertilization of bovine oocytes, Consult. Anim. Sci. 258: 10–15.Google Scholar
  29. Handrow, R. R., Lenz, R. W, and Ax, R. L., 1982, Structural comparisons among glycosaminoglycans to promote an acrosome reaction in bovine spermatozoa, Biochem. Biophys. Res. Commun. 107: 1326–1332.PubMedCrossRefGoogle Scholar
  30. Handrow. R. R., Boehm, S. K., Lenz, R. W, Robinson, J. A., and Ax, R. L., 1984, Specific binding of the glycosaminoglycan 3H-heparin to bovine, monkey and rabbit spermatozoa in vitro, J. Androl. 5: 51–63.Google Scholar
  31. Handrow, R. R., First, N. L., and Parrish, J. J., 1989, Calcium requirement and increased association with bovine sperm during capacitation by heparin, J. Exp. Zool. 252: 174–182.PubMedCrossRefGoogle Scholar
  32. Hiipakka, R. A., and Hammerstedt, R. H., 1978, 2-Deoxyglucose transport and phosphorylation by bovine sperm, Biol. Reprod. 19: 368.Google Scholar
  33. Hillery, F L., Parrish, J. J., and First, N. L., 1990, Bull specific effect on fertilization and embryo development in vitro, Theriogenology 33: 249.Google Scholar
  34. Hunter, A., and Moor, R. M., 1987, Stage dependent effects of inhibiting ribonucleic acids and protein synthesis on meiotic maturation of bovine oocytes in vitro, J. Diary Sci. 70: 1646–1652.CrossRefGoogle Scholar
  35. Hunter, R. H. F., and Wilmut, I., 1984, Sperm transport in the cow: Periovulatory redistribution of viable cells within the oviduct, Reprod. Nutr. Dev. 24: 597–608.PubMedCrossRefGoogle Scholar
  36. Lambert, R. D., Sirard, M. A., Bernard, C., Beland, R., Rioux, J. E., Leclerc, P., Menard, D. P, and Bedoya, M., 1985, In vitro fertilization of bovine oocytes matured in vivo and collected at laparoscopy, Theriogenology 25: 117–133.Google Scholar
  37. Langlais, J., and Roberts, K. D., 1985, A molecular membrane model of sperm capacitation and the acrosome reaction of mammalian spermatozoa, Gamete Res. 12: 183–224.CrossRefGoogle Scholar
  38. Langlais, J., Kan, F. W. K., Granger, L., Raymond, L., Bleau, G., and Roberts, K. D., 1988, Identification of sterol acceptors that stimulate cholesterol efflux from human spermatozoa during in vitro capacitation, Gamete Res. 20: 185–201.PubMedCrossRefGoogle Scholar
  39. Leclerc, P, Langlais, J., Lambert, R. D., Sirard, M. A., and Chafouleas, J. G., 1989, Effect of heparin on the expression of calmodulin-binding proteins in bull spermatozoa, J. Reprod. Fertil. 85: 615–622.PubMedCrossRefGoogle Scholar
  40. Lee, C. N., and Ax, R. L., 1984, Concentrations and composition of glycosaminoglycans in the female bovine reproductive tract, J. Diary Sci. 67: 2006–2009.CrossRefGoogle Scholar
  41. Lee, C. N., Handrow, R. R., Lenz, R. W, and Ax, R. L., 1985, Interactions of seminal plasma and glycosaminoglycans on acrosome reactions in bovine spermatozoa, Gamete Res. 12: 345–355.CrossRefGoogle Scholar
  42. Leibfried-Rutledge, M. L., Critser, E. S., and First, N. L., 1986, Effects of fetal calf serum and bovine serum albumin on in vitro maturation and fertilization of bovine and hamster cumulus-oocyte complexes, Biol. Reprod. 35: 850–857.PubMedCrossRefGoogle Scholar
  43. Leibfried-Rutledge, M. L., Critser, E. S., Parrish, J. J., and First, N. L., 1989, In vitro maturation and fertilization of bovine oocytes, Theriogenology 31: 61–74.Google Scholar
  44. Lenz, R. W, Ax, R. L., Grimek, H. J., and First, N. L., 1982, Proteoglycan from bovine follicular fluid enhances an acrosome reaction in bovine spermatozoa, Biochem. Biophys. Res. Commun. 106: 1092–1098.PubMedCrossRefGoogle Scholar
  45. Mahi-Brown, C. A., and Yanagimachi, R., 1983, Parameters influencing ovum pickup by oviductal fimbria in the golden hamster, Gamete Res. 8: 1–10.CrossRefGoogle Scholar
  46. Means, A. R., Tash, J. S., and Chafouleas, J. G., 1982, Physiological implications of the presence, distribution and regulation of calmodulin in eukaryotic cells, Physiol. Rev. 62: 1–38.PubMedGoogle Scholar
  47. Miller, D. J., 1989, The interaction between glycosaminoglycans and sperm and its regulation by seminal plasma, Ph.D. thesis, University of Wisconsin, Madison.Google Scholar
  48. Miller, D. J., and Ax, R. L., 1989, Chemical N-desulfation of heparin negates its ability to capacitate bovine spermatozoa, Gamete Res. 23: 451–465.PubMedCrossRefGoogle Scholar
  49. Motlik, J., and Fulka, J., 1986, Factors affecting meiotic competence in pig oocytes, Theriogenology 251: 87–96.CrossRefGoogle Scholar
  50. Murray, A. W., and Kirschner, M. W., 1989, Dominos and clocks: The union of two views of the cell cycle, Science 246: 614–621.PubMedCrossRefGoogle Scholar
  51. Niwa, K., and Ohgoda, O., 1988, Synergistic effect of caffeine and heparin on in-vitro fertilization of cattle oocytes matured in culture, Theriogenology 30: 733–741.PubMedCrossRefGoogle Scholar
  52. Niwa, K., Ohgoda, O., and Yuhara, M., 1988, Effects of caffeine in media for pretreatment of frozen-thawed sperm on in vitro penetration of cattle oocytes, in: Proceedings 11th International Congress of Animals Reproduction and Artificial Insemination, Vol. 3, International Congress of Animal Reproduction and Artificial Insemination, Dublin, pp. 346–348.Google Scholar
  53. Ohgoda, O., Niew, K., Yuhara, M., Takahashi, S., and Kanoya, K., 1988, Variation in penetration rates in vitro of bovine follicular oocytes do not reflect conception rates after artificial insemination using frozen semen from different bulls, Theriogenology 29: 1375–1381.CrossRefGoogle Scholar
  54. Oliphant, G., and Singhas, C. A., 1979, Iodination of rabbit sperm plasma membrane: Relationship of specific surface proteins to epididymal function and sperm capacitation, Biol. Reprod. 21: 937–944.PubMedCrossRefGoogle Scholar
  55. Olson, G. E., Winfrey, V. P., Garbers, D. L., and Noland, T. D., 1985, Isolation and characterization of a macromolecular complex associated with the outer acrosomal membrane of bovine spermatozoa, Biol. Reprod. 33: 761–779.PubMedCrossRefGoogle Scholar
  56. Park, C.-K., Ohgoda, O., and Niwa, K., 1989, Penetration of bovine follicular oocytes by frozen-thawed spermatozoa in the presence of caffeine and heparin, J. Reprod. Fertil. 86: 577–582.PubMedCrossRefGoogle Scholar
  57. Parrish, J. J., 1989, Capacitation of spermatozoa in vivo: Physiochemical and molecular mechanisms, J. Mol. Androl. 1: 87–111.Google Scholar
  58. Parrish, J. J., Susko-Parrish, J. L., and First, N. L., 1985, Effect of heparin and chondroitin sulfate on the acrosome reaction and fertility of bovine sperm in vitro, Theriogenology 24: 537–549.PubMedCrossRefGoogle Scholar
  59. Parrish, J. J., Susko-Parrish, J. L., Leibfried-Rutledge, M. L., Critser, E. S., Eyestone, W. H., and First, N. L., 1986, Bovine in vitro fertilization with frozen-thawed semen, Theriogenology 25: 591–600.PubMedCrossRefGoogle Scholar
  60. Parrish, J. J., Susko-Parrish, J. L., Winer, M. A., and First, N. L., 1988, Capacitation of bovine sperm by heparin, Biol. Reprod. 38: 1171–1180.PubMedCrossRefGoogle Scholar
  61. Parrish, J. J., Susko-Parrish, J. L., Handrow, R. H., Sims, M. M., and First, N. L., 1989a, Capacitation of bovine sperm by oviduct fluid, Biol. Reprod. 40: 1020–1025.PubMedCrossRefGoogle Scholar
  62. Parrish, J. J., Susko-Parrish, J. L., and First, N. L., 1989b, Capacitation of bovine sperm by heparin: Inhibitory effect of glucose and role of intracellular pH, Biol. Reprod. 41: 683–699.PubMedCrossRefGoogle Scholar
  63. Saeki, K., Leibfried-Rutledge, M. L., and First, N. L., 1990, Are fetal calf serum and hormones necessary during in vitro maturation of cattle oocytes for subsequent development? Theriogenology 33: 316.CrossRefGoogle Scholar
  64. Sato, E., Matsuo, M., and Miyamoto, H., 1990, Meiotic maturation of bovine oocytes in vitro: Improvement of meiotic competence by dibutyryl cyclic adenosine 3’,5’-monophosphate, J. Anim. Sci. 68: 1182–1187.PubMedGoogle Scholar
  65. Shur, B. D., 1989, Galactosyltransferase as a recognition molecule during fertilization and development, in: The Molecular Biology of Fertilization ( H. Schatten and G. Schatten, eds.), Academic Press, New York, pp. 37–71.Google Scholar
  66. Sirard, M. A., and Lambert, R. D., 1985, In vitro fertilization of bovine follicular oocytes obtained by laparoscopy, Biol. Reprod. 33: 487–494.Google Scholar
  67. Sirard, M. A., Lambert, R. D., Menard, D. P., and Bedoya, M., 1985, Pregnancies after in-vitro fertilization of cow follicular oocytes, their incubation in rabbit oviduct and their transfer to the cow uterus, J. Reprod. Fertil. 75: 551–556.PubMedCrossRefGoogle Scholar
  68. Sirard, M. A., Leibfried-Rutledge, M. L., Parrish, J. J., Ware, C. M., and First, N. L., 1988, The culture of bovine oocytes to obtain developmentally competent embryos, Biol. Reprod. 39: 546–552.PubMedCrossRefGoogle Scholar
  69. Sirard, M.. A., Florman, H. M., Leibfried-Rutledge, M. L., Barnes, F. L., Sims, M. L., and First, N. L., 1989, Timing of nuclear progression and protein synthesis necessary for meiotic maturation of bovine oocytes, Biol. Reprod. 40: 1257–1263.PubMedCrossRefGoogle Scholar
  70. Susko-Parrish, J. L., and Parrish, J. J., 1988, Glucose and cAMP modulators effect capacitation of bovine sperm by heparin, Biol. Reprod. 38 (Suppl. 1): 94.Google Scholar
  71. Susko-Parrish, J. L., Parrish, J. J., and Handrow, R. R., 1985, Glucose inhibits the action of heparin by an indirect mechanism, Biol. Reprod. 32 (Suppl. 1): 80.CrossRefGoogle Scholar
  72. Thibault, C., Gerard, M., and Menezo, Y., 1975, Preovulatory and ovulatory mechanisms in oocyte maturation, J. Reprod. Fertil. 45: 604–610.Google Scholar
  73. Tsafriri, A., Reich, R., and Abisogun, A. 0., 1987, The ovarian egg and ovulation, in: Marshall’s Physiology of Reproduction,Vol. III (G. E. Lemming, ed.), Churchill Livingstone, London (in press).Google Scholar
  74. Wassarman, P. M., 1987, Early events in mammalian fertilization, Annu. Rev. Cell Biol. 3: 109–142.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • J. J. Parrish
    • 1
  • N. L. First
    • 1
  1. 1.Department of Meat and Animal ScienceUniversity of WisconsinMadisonUSA

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