Synthesis, Characterization, and Use of New Solid and Hollow, Magnetic and Non-Magnetic, Organic-Inorganic Monodispersed Hybrid Microspheres

  • Shlomo Margel
  • Sigalit Gura
  • Hanna Bamnolker
  • Boaz Nitzan
  • Tami Tennenbaum
  • Beni Bar-Toov
  • Michael Hinz
  • H. Seliger
Chapter

Abstract

New organic-inorganic hybrid microspheres composed of cores of micron-sized monodispersed polystyrene microspheres (1–6 µm diameter) and shells of silica or iron oxide (Fe304-magnetite) microspheres of substantially lower diameter (30–40 nm) were formed by seeded polymerization of Si(OEt)4 or iron salts, respectively, on polystyrene microspheres. Leaching of traces of the magnetic coating, or iron salts, from the magnetic polystyrene shells into the working solution was prevented by a further coating with silica microspheres. Non-magnetic and magnetic hollow silica microspheres were formed by burning off the organic polystyrene core of the silica coated non-magnetic or magnetic polystyrene microspheres. Hybrid micro-spheres with functional groups other than inorganic hydroxyls were prepared by reacting the silica-coated particles with co-alcoholic reagents and/or co-alkylsilane compounds such as SiR3(CH2) X; R = Cl, OCH3, OC2H5, etc.; n = 3−17; X = CH3, CN, CO2Me, ΦCH2C1, etc. Particles containing ω-hydroxyl or primary amine groups were prepared by interacting the silica surfaces with alkylalkoxysilane compounds such as Si(OEt)3(CH2)3CO2CH3 or Si(OEt)3(CH2)3NH2; or with trichloroalkylsilane compounds such as SiC13(CH2)3CO2CH3 or SiC13(CH2)3CN, followed by diborane reduction of the terminal ester or nitrile surfaces, respectively. Polyaldehyde derivatized microspheres were formed by reacting the co-amine derivatized particles with polyacrolein microspheres of approximately 60 nm. Immuno-magnetic microspheres were prepared by covalent binding of the desired antibodies to the polyaldehyde particles. Preliminary studies demonstrated the potential use of the immuno-magnetic micro-spheres for two purposes concerning male infertility: (1) The specific removal of anti-sperm antibodies and sperm cells containing anti-sperm antibodies from semen of infertile males in vitro and (2) the separation of sperm cells from epithelial cells.

Keywords

Sperm Cell Magnetic Microsphere Polystyrene Microsphere Silica Microsphere Scanning Electron Microscopy Photomicrograph 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. 1.
    Rembaum A, Richard C and Glendale KY (1985). Hybride microspheres. U.S. patent 4, 534, 996.Google Scholar
  2. 2.
    Margel S and Weisel E (1984). Acrolein polymerization: Monodisperse homo and hybrido microspheres, synthesis, mechanism and reactions. J. Polymer Sci., Chem. Ed. 22, 145–158.Google Scholar
  3. 3.
    Philipse AP, van Bruggen MPB and Pathmamanoharan C (1994). Magnetic silica dispersions: Preparation and stability of surface-modified silica particles with a magnetic core. Langmuir 10, 92–99.CrossRefGoogle Scholar
  4. 4.
    Kawahashi N and Matijevic E (1990). Preparation and properties of uniform coated colloidal particles. V. Yttrium basic carbonate on polystyrene latex. J. Colloid and Interface Sci. 138, 534–542.CrossRefGoogle Scholar
  5. 5.
    Ohmori M and Matijevic E (1992). Preparation and properties of uniform coated colloidal particles. VII. Silica on hematite. J. Colloid and Interface Sci. 150, 594–597.CrossRefGoogle Scholar
  6. 6.
    Garg A and Matijevic E (1988). Preparation and properties of uniform coated inorganic particles. III. Zirconium hydrous oxide on hematite. J. Colloid and Interface Sci. 126, 243–250.CrossRefGoogle Scholar
  7. 7.
    Bamnolker H and Marge] S (1996). Dispersion polymerization of styrene in polar solvents: Effect of reaction parameters on microsphere surface and surface properties, size and size distribution and molecular weight. J. Polymer Sci., Chem. Ed. 34, 1857–1871.Google Scholar
  8. 8.
    Ober CK, Lok KP, Hair ML (1985). Monodispersed, micron-sized polystyrene particles by dispersion polymerization. J. Polym. Sci. Polym. Letters Edn. 23, 103–108.ADSCrossRefGoogle Scholar
  9. 9.
    Margel S and Bamnolker H (1995). Synthesis and use of new magnetic and non-magnetic solid and hollow microspheres. Patent pending (PCT, US12988).Google Scholar
  10. 10.
    Stober W, Fink A and Bohn E (1968). Controlled growth of monodispersed silica spheres in micron size range. J. Colloid and Interface Sci. 26, 62–69.CrossRefGoogle Scholar
  11. 11.
    Abe M, Itob T and Tamaura Y (1991). Preparation and application of magnetic films by ferrite plating in aqueous solution. Mat. Res. Soc. Symp. Proc. 232, 107–113.CrossRefGoogle Scholar
  12. 12.
    Zhang M, Zhang Q, ltoh T and Abe M (1994). Ferrite plating on porous silica microspheres for ultrasonic contrast agents. IEEE Transactions on Magnetics 30, 4692–4694.ADSCrossRefGoogle Scholar
  13. 13.
    Brandriss S and Marge] S (1993). Synthesis and characterization of self-assembled hydrophobic monolayer coatings onto silicon colloids. Langmuir 9, 1232–1240.CrossRefGoogle Scholar
  14. 14.
    Kawahashi N and Matijevic EJ (1991). Preparation of hollow spherical particles of yttrium compounds. J. Colloid and Interface Sci. 143, 103–110.CrossRefGoogle Scholar
  15. 15.
    Wikstrom P, Mandenius CF and Larsson PO (1988). Gas phase silylation. A rapid method for preparation of high-performance liquid chromatography supports. J. Chromatog. 455, 105–117.CrossRefGoogle Scholar
  16. 16.
    Dolitzky Y, Sturchak S, Nizan B, Sela BA and Marge] S (1994). Immobilized polyacrolein microspheres: Synthesis, characterization and their use as a new tool in diagnostics: A model, determination of antitrypsin in human serum. Analyt. Biochem. 220, 257–267.CrossRefGoogle Scholar
  17. 17.
    Bartoov B, Ben-Barak J, Mayevsky A, Mordechai S, Yogev L and Lightman A (1991). Sperm motility index: a new parameter for human sperm evaluation. Fertil. Steril. 56, 108–112.Google Scholar
  18. 18.
    Jager S, Kremer J and Van Slochteren-Draaisma T (1978). A simple method of screening for antisperm antibodies in the human male. Int. J. Fertil. 23, 12–21.Google Scholar
  19. 19.
    Alexander NJ (1989). Antibodies to sperm and infertility. In Andrology and Human Reproduction. Negro-Vilar A, Isidor A, Paulson J, Abdel Massih R and deCastro MPP (Eds.) New York, Raven Press. 47, 183–190.Google Scholar
  20. 20.
    Liu DY, Clarke GN and Baker HWG (1991). Inhibition of human sperm-zona pellucida and spermoolemma binding by antisperm antibodies. Fertil. Steril. 55, 440–442.Google Scholar
  21. 21.
    Bronson R, Cooper G and Rosenfeld D (1984). Sperm antibodies: their role in infertility. Fertil. Steril. 42, 171–183.Google Scholar
  22. 22.
    Fuchs EF and Alexander NJ (1983). Immunologic considerations before and after vasovasostomy. Fertil. Steril. 40, 497–499.Google Scholar
  23. 23.
    Bronson RA (1988). Immunobead binding. Present and future uses. In Perspectives in Immunoreproduction. Mathur S, Fredericks CM (Eds.) Hamsphere Publishing Corp. 147–159.Google Scholar
  24. 24.
    Kiser GC, Alexander NJ, Fuch EF and Fulgham DL (1987). In vitro immune absorption of antisperm. Fertil. Steril. 47, 466–474.Google Scholar
  25. 25.
    Wolff H and Anderson DJ (1988). Immunohistologic characterization and quantitation of leukocyte sub-populations in human semen. Fertil. Steril. 49, 497–504.Google Scholar
  26. 26.
    Smith DC, Barrat CLR and Williams MA (1989). The characterization of non-sperm cells in the ejaculates of fertile men using TEM. Andrologia 21, 319–333.CrossRefGoogle Scholar
  27. 27.
    Brody S, Gibbson WE and Lamb DJ (1991). Assisted reproductive techniques in the treatment of male infertility. In Infertility in the Male. Lipshultz LI and Hawards SS ( Eds. ), Second Edition, 427–447.Google Scholar
  28. 28.
    Lazar A, Silverstein L, Margel S and Mizrahi A (1985). Agarose-polyacrolein microsphere beads: a new microcarrier culturing system. Develop. Biolog. Stan. 60, 456–461.Google Scholar

Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Shlomo Margel
    • 1
  • Sigalit Gura
    • 1
  • Hanna Bamnolker
    • 1
  • Boaz Nitzan
    • 1
  • Tami Tennenbaum
    • 2
  • Beni Bar-Toov
    • 2
  • Michael Hinz
    • 3
  • H. Seliger
    • 3
  1. 1.Department of ChemistryIsrael
  2. 2.Department of Life ScienceBar-Ilan UniversityRamat-GanIsrael
  3. 3.Polymer SectionUniversity of UlmUlmGermany

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