Preparation and Application of Monosized Magnetic Particles in Selective Cell Separation

  • Wenche S. Prestvik
  • Arvid Berge
  • Preben C. Mørk
  • Per M. Stenstad
  • John Ugelstad
Chapter

Abstract

Some of the basic principles of formation of monosized macroporous particles by the method of “activated swelling” and the preparation of superparamagnetic particles based upon these particles are discussed. A short review of the applications of monodisperse, magnetic polymer particles in cell separation, with emphasis on recent work, is given. Some new work concerning non-specific adhesion of antibodies and cells on different beads, and the prevention of this unwanted phenomenon by help of casein are described. A method for the removal of excess magnetic beads by gradient centrifugation is presented. Positive cell separation, with easy liberation of free cells after magnetic isolation of rosetted cells, is obtained by use of particles with covalently coupled aminophenyl boronic acid at the surface.

Keywords

Magnetic Bead Polymer Particle Cell Separation Seed Particle Autologous Bone Marrow Transplantation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Wenche S. Prestvik
    • 1
  • Arvid Berge
    • 1
  • Preben C. Mørk
    • 1
  • Per M. Stenstad
    • 2
  • John Ugelstad
    • 1
  1. 1.Department of Industrial ChemistryNorwegian University of Science and TechnologyTrondheimNorway
  2. 2.SINTEF, Applied ChemistryTrondheimNorway

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