Site-Specific Cleavage of Insulin-Like Growth Factor II mRNAs
Insulin-like growth factor II (IGF-II) is a protein involved in growth and differentiation of numerous cell types. Because of the importance of IGF-II in many different processes, various regulatory mechanisms are available in the cell to control IGF-II protein synthesis, reflecting the vital importance of fine tuning and rapid regulation of IGF-II mRNA levels and IGF-II protein amount. One such mechanism is the site-specific endonucleolytic cleavage of IGF-II mRNAs, which will be described in this chapter. The role of an RNA-binding protein that is associated with the highly structured RNA will be discussed as well as the requirements for recognition of the IGF-II mRNA by an endoribonuclease.
KeywordsCleavage Site Cleavage Product Chicken Embryo Fibroblast Homeostatic Regulation Cleavage Efficiency
Unable to display preview. Download preview PDF.
- 5.Holthuizen, P.E., Steenbergh, P.H., and Sussenbach, J.S. 1999. Regulation of IGF gene expression. In The IGF System. Molecular Biology, Physiology, and Clinical Applications. R.G.Rosenfeld and Roberts,C.T., editors. Humana Press., Totowa, New Jersey, USA. 37–61.Google Scholar
- 15.Stoeckle, M.Y. and Hanafusa, H. 1989. Processing of 9E3 mRNA and regulation of its stability in normal and Rous sarcoma virus-transformed cells. Mo1.Cell Biol. 9: 4738–4745.Google Scholar
- 16.Binder, R., Horowitz, J.A., Basilion, J.P., Koeller, D.M., Klausner, R.D., and Harford, J.B. 1994. Evidence that the pathway of transferrin receptor mRNA degradation involves an endonucleolytic cleavage within the 3’ UTR and does not involve poly(A) tail shortening. EMBOJ. 13: 1969–1980.Google Scholar
- 17.Schlegl, J., Gegout, V., Schläger, B., Hentze, M.W., Westhof, E., Ehresmann, C., Ehresmann, B., and Romby, P. 1997. Probing the structure of the regulatory region of human transferrin receptor messenger RNA and its interaction with iron regulatory protein-1. Endocrinol.Metabol.Clin.North Am. 3: 1159–1172.Google Scholar