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Band Spreading in Chromatography

  • S. G. Perry
  • R. Amos
  • P. I. Brewer
Chapter

Abstract

Many outsiders with a scientific training looking in at the practice of chromatography must regard the exercise as an art or mystique, rather than as a science. There is no doubt that empiricism and experience frequently provide the dominating guidelines in setting up an experiment; theoretical considerations, even of a rudimentary nature, only infrequently playa major part in the design and operation of a chromatographic separation. This situation is even more pronounced in liquid chromatography than in the newer, but enormously more successfully applied technique of gas chromatography. Indeed, despite its seventy or eighty years of history, liquid chromatography has made very little progress, the materials and equipment in use today (at least for most adsorption work in columns) differing rather little from that of Day and Tswett.

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References

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Further Reading

  1. Knox, J. H., and Saleem, H., J. Chromatog. Sci. 7, 614 (1969). (Discusses kinetic conditions for optimum speed and resolution in column chromatography.)Google Scholar
  2. Smuts, T. W., van Niekerk, F. A., and Pretorius, V., J. Gas Chroma tog. 5, 190 (1967}). (Discusses the effect of operating parameters on speed of liquid chromatographCrossRefGoogle Scholar
  3. Huber, J. F. K., in Physical Separation Methods, Vol. IIB, Wilson, C. L., and Wilson, D. W., eds., Elsevier, Amsterdam Chapter 1, (1968). (Contains a presentation of the theory of liquid chromatography in columns.)Google Scholar

Copyright information

© Springer Science+Business Media New York 1973

Authors and Affiliations

  • S. G. Perry
    • 1
  • R. Amos
    • 1
  • P. I. Brewer
    • 1
  1. 1.Esso Petroleum CompanyLimited Esso Research Centre AbingdonBerkshireEngland

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