Liquid chromatography has an impressive history, stretching back into the 19th century, which has been well documented elsewhere(l). As an indispensable technique for the separation and analysis of organic substances it has made enormous strides in the past decade or so. Prior to 1958 column chromatography was conducted with comparatively large samples separated in, often short, almost invariably wide-bore, columns through which the developing liquid percolated under gravity. Thin-layer techniques were unknown outside a small group of workers(2), although paper chromatography was widely practiced.
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