A SDS/Page/Western Blot/EIA Protocol for the Specific Detection of Shrimp Viral Pathogens

  • Philip C. Loh
  • Lourdes M. Tapay
  • E. Cesar
  • B. NadalaJr.
  • Yuanan Lu
Chapter

Abstract

Shrimp viral diseases have seriously impacted the sustainability and economic success of the shrimp aquaculture industry worldwide. Among several of the recent viral pathogens which caused massive mortalities in cultured shrimp were the yellow-head virus (YHV) and the white spot baculovirus (WSBV) (also called the Chinese baculovirus [CBV] or the systemic ectodermal and mesodermal baculovirus [SEMBV]). Both YHV and CBV have been reported by our laboratory to be highly pathogenic for Penaeus stylirostris (blue shrimp) and P. vannamei (white shrimp), the two principal penaeid species commercially cultured in Hawaii and the Western Hemisphere (Lu et al. 1997). They are thus potentially a serious problem particularly to the brood stock industry.

Keywords

Penaeid Shrimp Confirmatory Assay Cell Culture Lysate Penaeid Prawn Specific Viral Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Philip C. Loh
    • 1
  • Lourdes M. Tapay
    • 1
  • E. Cesar
    • 1
  • B. NadalaJr.
    • 1
  • Yuanan Lu
    • 1
  1. 1.Department of MicrobiologyUniversity of HawaiiHonoluluUSA

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