Cellular and Molecular Requirements for B Lymphopoiesis
At certain levels, the subject of B lymphocyte differentiation is no longer a mystery. Molecular biological approaches have helped explain the generation of antibody diversity as well as the mechanisms of immunoglobulin gene regulation1. Nevertheless, some areas still remain fruitful for investigation. The bone marrow is the bursal equivalent in adult mammals. This is the site where B lymphocyte differentiation begins from a putatitive multipotent hemopoietic stem cell. With cells of at least eight different hematopoeitic lineages all confined within a calcified matrix, attempts to address the bone marrow as an organ system have been difficult at best. However, recent advances in methodology have provided an experimental system for in vitro modeling of the bone marrow microenvironment. This paper will focus on the recently characterized “stromal” cells which support B lymphopoiesis and their potential relevance to physiologic and pathologic processes.
KeywordsStromal Cell Neural Cell Adhesion Molecule Phosphatidyl Inositol Immunoglobulin Gene Bone Marrow Culture
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