Comparison of Wide-Field/Deconvolution and Confocal Microscopy for 3D Imaging

  • Peter J. Shaw

Abstract

Optical microscopy has always been a central technique to biological research, but in recent years its importance has vastly increased, mainly because of the introduction of epifluorescence imaging, which gives very sensitive detection, coupled with a multitude of highly specific fluorescent probes. Furthermore, it is often possible to obtain useful images noninvasively at light levels that are not damaging to living cells. Microinjection and other cell-loading methods can therefore be used in combination with fluorescence microscopy to analyze and modify subcellular structure and function in living cells.

Keywords

Spatial Frequency Focal Plane Point Spread Function Confocal Imaging Optical Section 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Peter J. Shaw
    • 1
  1. 1.Department of Cell BiologyJohn Innes InstituteColney Lane, NorwichUK

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