Expression of Ganglioside-Antigens During Neuronal Differentiation Studied by Use of Monoclonal Antibodies

  • H. Rösner
  • C. J. Willibald
  • S. Henke-Fahle
Part of the FIDIA Research Series book series (FIDIA, volume 6)

Abstract

Progress in development and maturation of the brain has been shown to be paralleled by changes of gangliosides (Rösner, 1982). One generalization of these studies was that predominance of GD3 may characterize mitotically active precursor cells (Dreyfus et al., 1975; Rösner, 1980; 1982; Rösner et al., 1985; Goldmann et al., 1984) and accretion of more complex gangliosides indicates the transition to postmitotic young neurons (Rösner, 1980; 1982). In the present study, performed with chicken and rat embryos, immunohistochemical data obtained with monoclonal antibodies to gangliosides support the above conclusion.

Keywords

Dorsal Root Ganglion Retinal Ganglion Cell Ventricular Zone Cortical Plate Postmitotic Neuron 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

TLC

thin chromatography

PB

sodium phosphate buffer

IgG-B

biotirylated antimousa IgG

IgG-F

fluoresciein-labeled antimouse IgG

A-F

fluoresciein-labeled avidin

PGAA

polysialoganglioside-associated antigen

DRG

dorsal root ganglion

VR

ventral root

GH

hexasialoganglioside.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1986

Authors and Affiliations

  • H. Rösner
    • 1
  • C. J. Willibald
    • 1
  • S. Henke-Fahle
    • 2
  1. 1.Institute of ZoologyUniversity of Stuttgart-HohenheimStuttgart 70Germany
  2. 2.Max-Planck-Institute for Developmental Biology7400 TübingenFederal Republic of Germany

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