A Structural Role for Dihydropteroyl Hexaglutamate in the Tail Baseplate of Various Bacteriophages

  • Lloyd M. Kozloff
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 163)

Abstract

A novel non-metabolic role is proposed for dihydropteroyl hexa-glutamate as a critical link binding together sub-structures of the tail of Escherichia coli bacteriophage T4. Six molecules of this folate compound have been found to be components of the complex tail baseplate of the phage particle. The baseplate is assembled using a total of at least 18 viral gene products in a series of reactions in which six wedge-like elements (each 0.7 × 106 daltons) bind symmetrically around a central tail plug (1.55 × 106 daltons) to form a flat hexagonal structure. It appears likely that the pteridine portion of the folate binds to a site on a viral-induced dihydro-folate reductase molecule, a wedge component, while the glutamate residues of the folate bind to a viral-induced thymidylate synthase molecule, a central plug component. Additionally, it appears that the folyl glutamate residues play a role in forming a flexible bond between the proximal end of the phage long tail fiber and the baseplate.

Keywords

Phage Particle Glutamate Residue Tail Fiber Heat Labile Flexible Bond 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Kozloff, L. M., and Lute, M., J. Mol. Biol., 12, 780–792 (1965).PubMedCrossRefGoogle Scholar
  2. 2.
    Edgar, R. S., and Wood, W. B., Proc. Nat. Acad. Sci. USA, 55, 498–505 (1966).PubMedCrossRefGoogle Scholar
  3. 3.
    Kikuchi, Y., and King, J., J. Mol. Biol., 99, 645–672 (1975).PubMedCrossRefGoogle Scholar
  4. 4.
    Kikuchi, Y., and King, J., J. Mol. Biol., 99, 673–694 (1975).PubMedCrossRefGoogle Scholar
  5. 5.
    Kikuchi, Y., and King, J., J. Mol. Biol., 99, 695–716 (1975).PubMedCrossRefGoogle Scholar
  6. 6.
    Kozloff, L. M.,SPI in: Bacteriophage assembly (M. Dubow, ed.), pp. 327–342, A. R. Liss, pub.Google Scholar
  7. 7.
    Simon, L. D., and Anderson, T. F., Virology, 32, 279–297 (1967).PubMedCrossRefGoogle Scholar
  8. 8.
    Simon, L. D., and Anderson, T. F., Virology, 32, 298–305 (1967).PubMedCrossRefGoogle Scholar
  9. 9.
    Nakamura, K., and Kozloff, L. M., Biochem. Biophys. Acta, 540, 313–319 (1978).PubMedCrossRefGoogle Scholar
  10. 10.
    Kozloff, L. M., Lute, M., and Crosby, L. K., J. Virol., 6, 754–759 (1970).PubMedGoogle Scholar
  11. 11.
    Kozloff, L. M., Crosby, L. K., and Baugh, C. M., J. Virol., 32, 497–506 (1979).PubMedGoogle Scholar
  12. 12.
    Kozloff, L. M., Lute, M., and Crosby, L. K., J. Virol., 23, 637–644 (1977).PubMedGoogle Scholar
  13. 13.
    Kozloff, L. M., Lute, M., and Crosby, L. K., SPI in: Chemistry and Biology of Pteridines, (R. L. Kisliuk and G. M. Brown, eds.), pp. 309–314, Developments in Biochemistry, Vol. 4, Elsevier/North Holland, New York (1978).Google Scholar
  14. 14.
    Kozloff, L. M., and Zorzopulos, J., J. Virol., 40, 635–644 (1981).PubMedGoogle Scholar
  15. 15.
    Kozloff, L. M., and Lute, M., J. Virol., 40, 645–656 (1981).PubMedGoogle Scholar
  16. 16.
    Kozloff, L. M., Lute, M., Crosby, L. K., Rao, N., Chapman, V. A., and DeLong, S. S., J. Virol., 5, 726–739 (1970).PubMedGoogle Scholar
  17. 17.
    Mosher, R. A., and Mathews, C. K., J. Virol., 31, 94–103 (1979).PubMedGoogle Scholar
  18. 18.
    Krauss, S. W., Stollar, B. D., and Friedkin, M., J. Virol., 11, 783–791 (1973).PubMedGoogle Scholar
  19. 19.
    Capco, G. R., Krupp, J. R., and Mathews, C. K., Arch. Biochem. Biophys., 158, 726–735 (1973).PubMedCrossRefGoogle Scholar
  20. 20.
    Kisliuk, R. L., Gaumont, Y., Baugh, C. M., Galivan, J. H., Maley, G. F., and Maley, F., SPI in: Chemistry and Biology of Pteridines (R. L. Kisliuk and G. M. Brown, eds.), pp. 431–436, Developments in Biochemistry, Vol. 4, Elsevier/North-Holland, New York (1978).Google Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • Lloyd M. Kozloff
    • 1
  1. 1.Department of Microbiology and ImmunologyUniversity of California, San FranciscoSan FranciscoUSA

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