Hydrophobic Chromatography and Its Potential Use in Biotechnology

  • S. Shaltiel

Abstract

Activation of agarose with CNBr (1) and subsequent coupling with alkylamines results in materials with new adsorption properties (2–7). Homologous series of such materials, identical in all respects except for the length of their attached hydrocarbon chains (Fig. 1), form the basis for a new approach to the resolution, purification and probing of proteins (2–5). This approach —“hydrophobic chromatography” — discriminates between proteins mainly through differences in the lipophilic interactions between the hydrocarbon chains and appropriately-sized hydrophobic pockets or patches in the various proteins.

Keywords

Glutamine Synthetase Hydrocarbon Chain Homologous Series Glycogen Phosphorylase Hydrophobic Chromatography 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    AXEN, R., PORATH, J. ERNBACK, S. Nature 224: 1302, 1967.CrossRefGoogle Scholar
  2. 2.
    ER-EL, Z., ZAIDENZAIG, Y. SHALTIEL, S. Biochem. Biophys. Res. Commun. 49: 383, 1972.Google Scholar
  3. 3.
    SHALTIEL, S. ER-EL, Z. Proc. Nati. Acad. Sci. USA 70: 778, 1973.CrossRefGoogle Scholar
  4. 4.
    SHALTIEL, S. Meth. Enzymol. 34: 126, 1974.CrossRefGoogle Scholar
  5. 5.
    SHALTIEL, S. FEBS Proc. 40:117, 1975.Google Scholar
  6. 6.
    YON, R.J. Biochem. J. Z26: 765, 1972.Google Scholar
  7. 7.
    HOFSTEE, B.H.J. OTILIO, N.F. Biochem. Biophys. Res. Commun. 50: 751, 1973.Google Scholar
  8. 8.
    SHALTIEL, S. in “Metabolic Interconversion of Enzymes - 1973” (Eds. E.H. Fischer, E.G. Krebs, H. Neurath and E.R. Stadtman) Springer-Verlag, New York, 1974, p. 379.Google Scholar
  9. 9.
    SHALTIEL, S., AMES, G.F. NOEL, K.D. Arch. Biochem. Biophys. Z59: 174, 1973.CrossRefGoogle Scholar
  10. 10.
    SHALTIEL, S. “Proc. 3rd Int. Symp. Metabolic Interconversion of Enzymes” p.62.Google Scholar
  11. 11.
    JAKUBOWSKI, H. PAWELKIEWICZ, J. FEBS Lett. 34: 150, 1973.CrossRefGoogle Scholar
  12. 12.
    PETERS, T., TANIUCHI, H. ANFINSEN, C.B. J. Biot. Chem. 246: 2447, 1973.Google Scholar
  13. 13.
    HOFSTEE, B.H.J. Anal. Biochem. 52: 430, 1973.CrossRefGoogle Scholar
  14. 14.
    SHALTIEL, S., HENDERSON, G.B. SNELL, E.E. Biochemistry 13:Google Scholar
  15. 15.
    HENDERSON, G.B., SHALTIEL, S. ö SNELL, E.E. Biochemistry 13:Google Scholar
  16. 16.
    ER-EL, Z. SHALTIEL, S. FEBS Lett. 40: 142, 1974.Google Scholar
  17. 17.
    HJERTEN, S. J. Chromatogr. 87: 325, 1973.CrossRefGoogle Scholar
  18. 18.
    PORATH, J., SUNDBERG, L., FORNSTEDT, N. OLSEN, J. Nature 245: 465, 1973.CrossRefGoogle Scholar
  19. 19.
    RIMERMAN, R.A. HATFIELD, G.W. Science Z82: 383, 1973.Google Scholar
  20. 20.
    YON, R.J. Biochem. J. Z37: 127, 1974.Google Scholar
  21. 21.
    HOKSE, H. Carbohyd. Res. 37: 390, 1974.CrossRefGoogle Scholar
  22. 22.
    JENNISSEN, H.P. HEILMEYER, L.M.G. Biochemistry Z4: 754, 1975.Google Scholar
  23. 23.
    THANNER, F., PALM, D. SHALTIEL, S. FEBS Lett. 55: 178, 1975.CrossRefGoogle Scholar
  24. 24.
    SHALTIEL, S. ADLER, S.P., PURICH, D., KABAN, C., SENIOR, P. STADTMAN, E.R. Proc. NatZ. Acad. Sci. USA 72: 3397, 1975.CrossRefGoogle Scholar
  25. 25.
    RAIBAUD, O., HOGBERG-RAIBAUD, A. GOLDBERG, M. E. FEBS Lett. 50: 130, 1975.CrossRefGoogle Scholar
  26. 26.
    ER-EL, Z. Ph.D. thesis, Weizmann Institute of Science, Israel, 1975.Google Scholar
  27. 27.
    KULA, M.R., HATEF-HAGHI, D., TAUBER-FINKELSTEIN, M. SHALTIEL, S. Biochem. Biophys. Res. Commun. 69: 389, 1976.CrossRefGoogle Scholar
  28. 28.
    DAVEY, M.W., HUANG, J.W., SULKOWSKI, E. CARTER, W.A. J. Biol. Chem. 250: 348, 1975.Google Scholar
  29. 29.
    SHALTIEL, S., HEDRICK, J.L. FISHCER, E.H. Biochemistry 5:Google Scholar
  30. 30.
    HEDRICK, J.L., SHALTIEL, S. FISCHER, E.H. Biochemistry 8:Google Scholar
  31. 31.
    AMSTERDAM, A., ER-EL, Z. SHALTIEL, S. Israel J. Med. Sci. ZO: 1580, 1974.Google Scholar
  32. 32.
    AMSTERDAM, A., ER-EL, Z. SHALTIEL, S. Arch. Biochem. Biophys. Z71: 673, 1975.CrossRefGoogle Scholar
  33. 33.
    CUATRECASAS, P., WILCHEK, M. ANFINSEN, C. B. Proc. NatZ. Acad. Sci. USA, 61; 636, 1968.CrossRefGoogle Scholar
  34. 34.
    JAKOBY, W.B. WILCHEK, M. (Eds.) Meth. Enzymol. 34:1974.Google Scholar

Copyright information

© Springer Science+Business Media New York 1978

Authors and Affiliations

  • S. Shaltiel
    • 1
  1. 1.Department of Chemical ImmunologyThe Weizmann Institute of ScienceRehovotIsrael

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