Scale-Up of Enzyme Purification

  • Henry R. Bungay

Abstract

In general, enzymes now sold for industrial uses are very crude compared to those sold for research or for medical purposes. Eventually, the demand for pure enzymes will climb as medical applications increase and more highly purified grades are needed in bulk quantities for immobilization and for industrial situations where better process control and fewer side reactions are important. Current processes for enzyme purification make extensive use of gel chromatography, an operation poorly suited for large scale application because of a serious deterioration of performance as the size of the column is increased. Either such steps must be improved dramatically or alternatives must be found. This paper assesses some purification technology and possible future trends in this field.

Keywords

Ammonium Sulfate Polyethylene Glycol Enzyme Purification Affinity Matrix Pure Enzyme 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    HSU, L.H., FLORA, R.M.& BUNGAY, H.R. 3rd. Enzyme Technology Digest 4: 4, 1975.Google Scholar
  2. 2.
    JANSON, J.C., ERSSON, B.& PORATH, J. Biotechnol. Bioengr. 16: 21, 1974.CrossRefGoogle Scholar
  3. 3.
    ALBERTSSON, P.A., “Partition of Cell Particles and Macromolecules” 2nd Ed., Wiley-Interscience, New York, 1971.Google Scholar
  4. 4.
    JOHANSSON, G., HARTMAN, A.& ALBERTSSON, P.A. Eur. J. Biochem. 33: 379, 1973.CrossRefGoogle Scholar
  5. 5.
    LILLY, M.D. & DUNNILL, P. In “Fermentation Advances” (Ed. D Perlman) Academic Press, New York, 1969, p. 225.Google Scholar
  6. 6.
    DUNNILL, P. & LILLY, M.D. In “Enzyme Engineering” (Ed. L.B. Wingard, Jr.) Wiley-Interscience, New York, 1972, p. 97.Google Scholar
  7. 7.
    DUNNILL, P. & LILLY, M.D. In “Enzyme Engineering, Vol. 2” (Ed. E.K. Pye and L.B. Wingard, Jr.) Plenum Press, New York, 1974. p. 43.Google Scholar

Copyright information

© Springer Science+Business Media New York 1978

Authors and Affiliations

  • Henry R. Bungay
    • 1
  1. 1.Worthington Biochemical CorporationFreeholdUSA

Personalised recommendations