Unit Operations of Enzyme Purification

  • C. W. Hancher

Abstract

The successful purification of an enzyme from a bacterial or tissue source usually follows a general separation flowsheet consisting of the following general processing steps: (1) Starting material preparation and cell harvesting, which includes tissue removal, grinding, cellular disruption and centrifugation to produce a crude extract; (2) Crude extract separation, which includes DEAE, or other charged support, either fractional or total product precipitation, or product separation by size, gel exclusion, or membrane sizing; (3) Secondary separation of the partially purified product, which usually is a combination of the above separation steps; (4) Pre- and post-treatment of the product (e.g. salt removal, water removal, and pH adjustment) to prepare for the next separation operation, which is frequently time-consuming and results in a possibility of product loss.

Keywords

Hydraulic Head DEAE Cellulose Water Removal DEAE Column Salt Removal 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1978

Authors and Affiliations

  • C. W. Hancher
    • 1
  1. 1.Chemical Technology DivisionOak Ridge National LaboratoryOak RidgeUSA

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