Endotoxin pp 347-359 | Cite as

Possible Refractory Site on LPS-Induced Interleukin 1 Production in C3H/HeJ Peritoneal Macrophages

  • M. Nakano
  • Y. Terada
  • H. Matsumura
  • H. Shinomiya
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 256)

Abstract

The C3H/HeJ strain of mice is known to be unresponsive to LPS (20), and this peculiar characteristic of these mice has greatly contributed to the analysis of the complex processes involved in the cell activation by LPS as a negative control. The unresponsiveness of these mice is believed to be attributed to a mutation of a single gene locus on chromosome 4 that has been designated as the LPS-gene with normal (n) and defective (d), alleles, respectively (26). Because C3H/HeJ mice carry the LPS-defective gene (Lpsd), their macrophages cannot secrete interleukin 1 (IL-1) in response to LPS (21). Determining the location of where the blocking sites are phenotypically expressed by the defective gene may provide us with a useful approach for elucidating the triggering by LPS, because it must be one of the important sites of this pathway. In a previous paper (22), we demonstrated that the C3H/HeJ macrophages are unresponsive to the calcium ionophore A23187 as well as LPS, ang we suggested that the blocking sites expressed phenotypically by the Lps are shared by LPS- and A23187-stimulated processes. In the present study, we intend to elucidate the difference of intracellular signal transmission on the LPS-induced IL-1 production in macrophages between LPS-responsive C3H/He mice and LPS-unresponsive C3H/HeJ mice.

Keywords

Calcium Ionophore Peritoneal Exudate Cell Calcium Ionophore A23187 Human Peripheral Blood Monocyte Blocking Site 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. 1.
    Castagna, M. Y., Takai, Y., Kaibuchi, K., Sano, K., Kikkawa, U. and Nishizuka, Y., 1982, Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol ester. J. Biol. Chem. 257: 7847.Google Scholar
  2. 2.
    Cheung, W. Y., 1980, Calmodulin plays a pivotal role in cellular regulation. Science 207: 19.PubMedCrossRefGoogle Scholar
  3. 3.
    Chirgwin, J. M., Przybyla, A. E., Macdonald, R. J. and Rutter, W. J., 1979, Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18: 5294.PubMedCrossRefGoogle Scholar
  4. 4.
    Fenton, M. J., Clark, B. D., Collins, K. L., Webb, A. C., Rich, A. and Auron, P. E., 1987, Transcriptional regulation of the human prointerleukin lb gene. J. Immunol. 138: 3972.PubMedGoogle Scholar
  5. 5.
    Hidaka, H., Inagaki, M., Kawamoto, S. and Sasaki, Y., 1984, Isoqunolinesulfonamides, novel and potent inhibitors of cyclic nucleotide dependent protein kinase and protein kinase C. Biochemistry 23: 5036.PubMedCrossRefGoogle Scholar
  6. 6.
    Hidaka, H., Sasaki, Y., Tanaka, T., Endo, T., Ohno, S., Fujii, Y. and Nagata, T., 1981, N-(6-aminopexyl)-5-chloro-l-naphthalenesulfonamide, a calmodulin antagonist, inhibits cell proliferation. Proc. Natl. Acad. Sci. U.S.A. 78: 4354.Google Scholar
  7. 7.
    Kakiuchi, S., Yasuda, S., Yamazaki, R., Teshima, Y., Kanada, K. and Soube, K., 1982, Quantitative determinations of calmodulin in the supernatant and particulate fractions of mammalian tissues. J. Biochem. 92: 1041.PubMedGoogle Scholar
  8. 8.
    Koide, S. and Steinman, R. M., 1987. Induction of murine interleukin 1: stimuli and responsive primary cells. Proc. Natl. Acad. Sci. U.S.A. 84: 3 802.Google Scholar
  9. 9.
    Laporte, D. C. and Strom. D. R., 1978, Detecton of calcium-dependent regulatory protein binding components using “31-labeled calcium-dependent regulatory protein. J. Biol. Chem. 253: 3347.Google Scholar
  10. 10.
    Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J., 1951, Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265.Google Scholar
  11. 1l.
    Maniatis, T., Fritsch, E. F. and Sambrook, J., 1982, “Molecular Cloning,” Cold Spring Harbor Laboratory, New York.Google Scholar
  12. 12.
    March, C. J., Mosley, B., Larsen, A., Cerretti, P., Braedt, G., Price, V., Gillis, S.. Henney, C. S., Kronheim, S. R., Grabstein, K., Conlon, P. J., Hopp, T. P. and Cosman, D.. 1985, Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs. Nature 315: 641.PubMedCrossRefGoogle Scholar
  13. 13.
    Matsushima, K., Taguchi, M., Kovaks, E. J., Young, H. A. and Oppenheim, J. J., 1986, Intracellular localization of human monocytes associated interleukin 1 activity and release of biologically active IL-1 from monocytes by trypsin and plasmin. J. Immunol. 136: 2883.PubMedGoogle Scholar
  14. 14.
    Matsushima, K. and Oppenheim, J. J., 1985. Calcium ionophore (A23187) increases interleukin 1 (IL 1) production by human peripheral blood monocytes and interacts synergistically with IL 1 to augment concanavalin A-stimulated thymocyte proliferation. Cell. Immunol. 90: 226.Google Scholar
  15. 15.
    Mizel, S. B., Oppenheim, J. J. and Rosenstreich, D. L., 1978, Characterization of lymphocyte-activating factor (LAF) produced by the macrophage cell line, P388D1. I. Enhancement of LAF production by activated T lymphocytes. J. Immunol. 120: 1497.Google Scholar
  16. 16.
    Nishikawa, M., Tanaka, T. and Hidaka. H., 1980, Ca2+-calmodulindependent phosphorylation and platelet secretion. Nature 287: 863.PubMedCrossRefGoogle Scholar
  17. 17.
    ppenheim, J. J., Stadler, B. M., Siraganian, R. P., Mage, M. and Mathieson. B., 1982, Lymphokines: Their role in lymphocyte responses. Properties of interleukin 1. Fed. Proc. 47: 257.Google Scholar
  18. 18.
    Orlow, S. J., Rosenstreich, D. L., Pifco-Hirst, S. and Rosen, 0. M., 1985, Purification and distribution of a novel macrophage-specific calmodulin-binding glycoprotein. J. Immunol. 134: 449.PubMedGoogle Scholar
  19. 19.
    Prpic, V., Weiel, J. E., Somers, S. D., DiGuiseppi, J., Gonias, S. L., Pizzo, S. V., Hamilton, T. A., Herman, B. and Adams, D. O., 1987, Effects of bacterial lipopolysaccharide on the hydrolysis of phosphatidylinosotol-4.5-bisphosphate in murine peritoneal macrophages. J. Immunol. 139: 526.PubMedGoogle Scholar
  20. 20.
    Rosenstreich, D. L., 1985, Genetic control of endotoxin response: C3H/HeJ mice, in: “Handbook of Endotoxin,” L. J. Berry, ed., Elsevier/North-Holland Biomedical Press, New York. 3: 82.Google Scholar
  21. 21.
    Rosenstreich, D. L., Vogel, S. N., Jacques, A. R., Wahl, L. M. and Oppenheim, J. J., 1978, Macrophage sensitivity to endotoxin: genetic control by a single codominant gene. J. Immunol. 121: 1664.PubMedGoogle Scholar
  22. 22.
    Shinomiya H. and Nakano, M., 1987. Calcium ionophore A23187 does not stimulate lipopolysaccharide nonresponsive C3H/HeJ peritoneal macrophages to produce interleukin 1. J. Immunol. 139: 2730.PubMedGoogle Scholar
  23. 23.
    Soube, K., Yamazaki, R., Yasuda, S. and Kakiuchi, S., 1981, Identity of the particulate form of calmodulin with soluble calmodulin. FEBS Lett. 129: 215.CrossRefGoogle Scholar
  24. 24.
    Speaker, M. G., Sturgill, T. W., Orlow, S. J.. Chia, G. H., PifckoHirst, S. and Rosen, O. M., 1980, The effects of trifluoperazine on the macrophage-like cell line, J774. Ann. N. Y. Acad. Sci. 356: 162.Google Scholar
  25. 25.
    Speaker, M. G., Orlow, S. J., Sturgill, T. W. and Rosen, 0. M., 1983. Characterization of a calmodulin-binding protein that is deficient in trifluoperazine-resistant variants of the macrophage-like cell line J774. Proc. Natl. Acad. Sci. U.S.A. 88: 329.Google Scholar
  26. 26.
    Watson, J., Kelly, K. Largen, M. and Taylor, B. A., 1978. The genetic mapping of a defective LPS response gene in C3H/HeJ mice. J. Immunol. 120: 422.Google Scholar
  27. 27.
    Westphal, O. and Luderitz, 0., 1954, Chemische erforschung von lipopolysacchariden gram-negative bakterien. Angew. Chem. 66: 407.Google Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • M. Nakano
    • 1
  • Y. Terada
    • 1
  • H. Matsumura
    • 1
  • H. Shinomiya
    • 1
  1. 1.Department of MicrobiologyJichi Medical SchoolTochigiJapan

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