Endotoxin pp 3-11 | Cite as

Chemical Synthesis of Endotoxin

  • S. Kusumoto
  • N. Kusunose
  • M. Imoto
  • T. Kamikawa
  • T. Shiba
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 256)

Abstract

Total synthesis of lipid A’s of Escherichia coli and many other bacterial species achieved in our laboratory (6, 7, 8) contributed not only to confirm their proposed chemical structures but also to establish unequivocally that these phosphorylated polyacyl glucosamine disaccharide with definite structures are responsible for most of the endotoxic activities of bacterial lipopolysaccharide (LPS) (4, 5). Furthermore, precise study on the biological activities could be carried out in relation to the acylation or phosphorylation patterns of the molecules with these pure synthetic preparations of lipid A’s in hand (12, 13). Immunogenicity and antigenicity of lipid A could be analyzed as well (1, 2). As the results, evidences were obtained which indicated that the toxic and other biological activities of endotoxin could be separated by adequate modification of the structure such as distribution of the acyl groups. One might thus be able to utilize some of the beneficial biological activities by further research in this line.

Keywords

Boron Trifluoride Lithium Aluminum Hydride Boron Trifluoride Etherate Synthetic Lipid Centrifugal Partition Chromatography 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • S. Kusumoto
    • 1
  • N. Kusunose
    • 1
  • M. Imoto
    • 1
  • T. Kamikawa
    • 1
  • T. Shiba
    • 1
  1. 1.Department of Chemistry, Faculty of ScienceOsaka UniversityToyonaka Osaka 560Japan

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