Serial Changes in Cytosolic, Mitochondrial, and Lysosomal Enzymes and Cardiac Myosin Light Chain II in Plasma following Coronary Ligation in Conscious Closed-Chest Dogs
We studied serial changes in various myocardial enzymes and cardiac myosin light chain II (LCII) in plasma following coronary ligation in 14 conscious closed-chest dogs. Cytoplasmic enzymes [creatine Phosphokinase (CPK) and supernatant glutamic oxaloacetic transaminase (sGOT)] reached maximum at 12–24 hr and returned to normal at 72–96 hr. The mitochondrial isozyme of GOT (mGOT) began to rise at 6–9 hr, peaked at 12–30 hr (4.8–42.2 IU/liter), and stayed higher at 96 hr than before infarction. Glutamate dehydrogenase (GLDH), another mitochondrial enzyme, began to elevate at 6–16 hr and reached maximum at 24–60 hr (6.2–20.5 U/liter); GLDH also showed higher levels at 96 hr than before infarction. N-Acetyl-ß-glucosaminidase (NAG), a lysosomal enzyme, showed a biphasic pattern in every case. The first peak appeared at 3–12 hr, and the second one at 36–72 hr. Myosin LCII began to rise at 3–9 hr, peaked at 30–120 hr (34–136 ng/ml), and remained elevated for 7 to 10 days. Determination of these myocardial enzymes or LCII in plasma is useful for the diagnosis of acute myocardial infarction.
KeywordsAcute Myocardial Infarction Lysosomal Enzyme Glutamate Dehydrogenase Creatine Phosphokinase Plasma Activity
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