Pathogenesis of Immune-Mediated Carditis in Monkeys

  • I. S. Anand
  • N. K. Ganguly
  • A. K. Khanna
  • R. N. Chakravarti
  • P. L. Wahi

Abstract

An experimental model of carditis has been produced in the rhesus monkey by giving 12 weekly injections of streptococcal membrane antigen. Carditis was produced in as short a period as 14 weeks. There was evidence of myocarditis, endocarditis, and, in two animals, myocardial granuloma formation. No valvular lesions were seen. Measurement of immune responses showed that heart cross-reactive antibodies started appearing in the circulation after the second injection. By the sixth injection, there was evidence of complement consumption and appearance of circulating immune complexes. Antibody-dependent cell cytotoxicity started operating after the second injection, and by the sixth injection, peripheral lymphocytes had acquired hypersensitivity to membrane antigen. It is concluded that some of these immunologic responses might have played a role in the genesis of carditis.

Keywords

Rhesus Monkey Rheumatic Fever Rheumatic Heart Disease Streptococcal Infection Acute Rheumatic Fever 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Chaturvedi, U. C., Davies, J. W., and Flewett, T. H. 1973. Separation and characterization of cardiac antigen proteins. Clin. Exp. Immunol. 15:613–622.PubMedGoogle Scholar
  2. 2.
    Cromarti C. W. J., and Craddock, J. G. 1966. Rheumatic-like cardiac lesions in mice. Science154:285–287.CrossRefGoogle Scholar
  3. 3.
    Eckhardt, R., Kloos, P., Dierich, M. P., Meyer, Z., and Buschenfelde, K. H. 1977. K-lymphocytes (killer cells) in Crohn’s disease and acute virus B-hepatitis. Gut. 18:1010–1016.PubMedCrossRefGoogle Scholar
  4. 4.
    Fürth, R., Van Zwet, T. L., and Leijh, P. C. J. 1978. In vitrodetermination of phagocytosis and intracellular killing by polymorphonuclear and monophagocytes. In: D. M. Weir (ed.), Handbook of Experimental Immunology, pp. 31.1–32.19. Blackwell ScientifiC., Oxford.Google Scholar
  5. 5.
    Ganguly, N. K., Chugh, K. S., Pal, Y., and Sapru, R. P. 1977. Comparative values of C’3 in normal rhesus monkeys. Indian J. Med. Res. 66:570–575.PubMedGoogle Scholar
  6. 6.
    Ganguly, N. K., Sapru, R. P., and Mohan, C. 1977. Comparative evaluation of anti-hya-luronidase, antistreptolysin O and streptozyme tests in acute rheumatic activity. Indian J. Med. Res. 66:802–808.PubMedGoogle Scholar
  7. 7.
    Goldstein, I., Halpern, B., and Robert L. 1967. Immunological relationship between streptococcus A polysaccharide and the structural glycoproteins of the heart valves. Nature213:44–47.CrossRefGoogle Scholar
  8. 8.
    Grant, J. 1978. Immunological methods in bacteriology. In: D. M. Weir (ed.), Handbook of Experimental Immunology, pp. 39.1–39.15. Blackwell ScientifiC., Oxford.Google Scholar
  9. 9.
    Hess, E. V., Fink, C. V., Taranta, A., and Ziff, M. 1964. Heart muscle antibodies in rheumatic fever and other diseases. J. Clin. Invest. 43:886–893.PubMedCrossRefGoogle Scholar
  10. 10.
    Kaplan, M. H. 1965. Autoantibodies to heart and rheumatic fever, the induction of autoimmunity to heart by streptococcal antigen cross reactive with heart. Ann. N.Y. Acad. Sci. 124:904–915.PubMedCrossRefGoogle Scholar
  11. 11.
    Kaplan, M. H. 1965. Induction of acute autoimmunity to heart in rheumatic fever by streptococcal antigen(s). Cross reaction with heart. Fed. Proc. 24:109–112.PubMedGoogle Scholar
  12. 12.
    Kaplan, M. H., Bolande, R., and Rakita, L. 1964. Presence of bound immunoglobulins and complement in the myocardium in acute rheumatic fever. N. Engl. J. Med. 271:637–645.PubMedCrossRefGoogle Scholar
  13. 13.
    Kaplan, M. H., and Meyserian, M. 1962. An immunological cross reaction between group A streptococcal cells and human heart tissue. Lancet1:706–710.PubMedCrossRefGoogle Scholar
  14. 14.
    Kaplan, M. H., and Suce, K. H. 1964. Immunological relation of streptococcal and tissue antigens III. J. Exp. Med. 119:651–666.PubMedCrossRefGoogle Scholar
  15. 15.
    Mohan, C. 1979. Pathogenecity of Group A Beta Haemolytic Streptococcus and Its L-Forms in Rhesus Monkeys. Ph.D. Thesis, Postgraduate Institute of Medical Education and Research, Chandigarh, India.Google Scholar
  16. 16.
    Mohan, C., Ganguly, N. K., Chakravarti, R. N., and Chitkara, N. L. 1977. Prevalence of haemolytic streptococcal infection in rhesus monkeys. J. Hyg. Epidemiol. Microbiol. Immunol. (Praha)21:203–208.Google Scholar
  17. 17.
    Murphy, G. E., and Swift, H. F. 1950. The induction of rheumatic like cardiac lesions in rabbits by repeated focal infection with group A streptococci. Comparison with the cardiac lesions of serum disease. J. Exp. Med. 91:485–498.PubMedCrossRefGoogle Scholar
  18. 18.
    Ohanion, S. H., and Schwab, J. H. 1967. Persistence of group A streptococcal cell walls related to chronic inflammation of rabbit dermal connective tissue. J. Exp. Med. 125:1137–1148.CrossRefGoogle Scholar
  19. 19.
    Pichler, W. J., and Krupp, M. 1977. Receptors of IgM coated erythrocytes on chronic lymphocytic leukemia cells. J. Immunol. 118:1010–1015.PubMedGoogle Scholar
  20. 20.
    Quan, P. C., and Burtin, P. 1978. E-rosette forming cells at 29°C: An assay for the immune status of cancer patients. J. Cancer38:606–611.CrossRefGoogle Scholar
  21. 21.
    Qurrie, G. A., and Hedley, D. W. 1977. Monocyte and macrophage in malignant melanoma. Peripheral blood macrophage precursors. Br. J. Cancer36:1–6.CrossRefGoogle Scholar
  22. 22.
    Read, S. R., Fischetti, V. A., Utermohlen, V., Falk, R. E., and Zabriskie, J. B. 1974. Cellular reactivity studies to streptococcal antigens. Migration inhibition studies in patients with streptococcal infections and rheumatic fever. J. Clin. Invest. 54:439–450.PubMedCrossRefGoogle Scholar
  23. 23.
    Rotta, J., and Tawil, G. S. 1976. Manual of Reference Procedures in Streptococcal Bacteriology and Serology. WHO Technical Report, World Health Organization, Geneva.Google Scholar
  24. 24.
    Sapru, R. P., Ganguly, N. K., Sharma, S., Chandnani, R. E., and Gupta, A. K. 1977. Cellular reactions to group A beta haemolytic streptococcal membrane antigen and its relation to complement levels in patients with rheumatic heart disease. Br. Med. J. 2:422–424.PubMedCrossRefGoogle Scholar
  25. 25.
    Stollerman, G. H. 1975. Rheumatic Fever and Streptococcal Infection. Grune &; Stratton, New York.Google Scholar
  26. 26.
    Taranta, A. 1972. Rheumatic fever: Clinical aspects, In: J. L. Hollander and D. J. McCarty, (eds.), Arthritis and Allied Conditions, pp. 764–820. Lea & Febiger, Philadelphia.Google Scholar
  27. 27.
    Taranta, A., Cuppari, G., and Quagliata, F. 1969. Dissociation of hemolytic and lymphocyte transforming activities of streptolysin preparation. J. Exp. Med. 129:605–622.PubMedCrossRefGoogle Scholar
  28. 28.
    Thomas, E. 1952. Rheumatic Fever: A Symposium. University of Minnesota Press, Minneapolis.Google Scholar
  29. 29.
    Varier Jones, J., and Cumming, R. N. 1977. Test for circulating immune complexes. In: R. A. Thompson (ed.), Techniques of Clinical Immunology, pp. 136–156. Blackwell ScientifiC., Oxford.Google Scholar
  30. 30.
    Van de Rijn, I., Zabriskie, J. B., and McCarty, M. 1977. Group A streptococcal antigens cross reactivity with myocardium purification of HRA and isolation and characterization of streptococcal antigen. J. Exp. Med. 146:579–599.PubMedCrossRefGoogle Scholar
  31. 31.
    Yang, L. C., Soprey, P. R., Wittner, M. K., and Fox, E. N. 1977. Streptococcal induced cell mediated immune destruction of cardiac myofibres in vitro. J. Exp. Med. 146:344–360.CrossRefGoogle Scholar
  32. 32.
    Zabriskie, J. B., and Freimer, E. H. 1966. An immunological relationship between the group A streptococcus and mammalian muscle. J. Exp. Med. 124:661–678.PubMedCrossRefGoogle Scholar
  33. 33.
    Zimmerman, R. A., Krushan, D. H., Wilson, E., and Douglas, J. D. 1970. Human streptococcal disease syndrome compared with observations in chimpanzees, III. Immunologic responses to induced pharyngitis and the effect of treatment. J. Infect. Dis. 122:280–289.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1983

Authors and Affiliations

  • I. S. Anand
    • 1
  • N. K. Ganguly
    • 1
  • A. K. Khanna
    • 1
  • R. N. Chakravarti
    • 1
  • P. L. Wahi
    • 1
  1. 1.Departments of Cardiology, Parasitology, and Experimental MedicinePostgraduate Institute of Medical Education and ResearchChandigarhIndia

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