Optical Imaging and In Vivo Microscopy
Useful images of hidden structures or pathological lesions can be created with X-rays, ultrasound, radioactive tracer, and magnetic resonance. However, there are many biological processes that cannot be easily or directly monitored by using computed tomography (CT), magnetic resonance imaging (MRI), or nuclear imaging because key molecules in these processes are not distinguishable from each other with these imaging techniques. Light penetrates tissue and is reflected at the surface. Whatever light does penetrate tissue is scattered widely so that shadows of internal structures are blurred beyond recognition. Light has been used in all the varieties of endoscopic imaging, where rigid or flexible tubes are inserted through incisions or natural body openings.
KeywordsGreen Fluorescent Protein Portal Vein Optical Coherence Tomography Hepatic Artery Kupffer Cell
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- 3.Hebden JC, Delpy DT. Diagnostic imaging with light. Br J Radiol 1997;70:S206–S214.Google Scholar
- 7.McCuskey RS. Microscopic methods for studying the microvasculature of internal organs. In: Baker CH, Nastuk WF, eds. Physical Techniques in Biology and Medicine: Microvascular Technology. New York: Academic Press, 1986:247–264.Google Scholar
- 8.Grisham JW, Nopanitaya W. Scanning electron microscopy of casts of hepatic microvessels. Review of methods and results. In: Lautt WW, ed. Hepatic Circulation in Health and Disease. New York: Raven Press, 1981:87–109.Google Scholar
- 10.Charnsangavej C, Wallace S. Interventional radiologic techniques in the diagnosis and treatment of hepatobiliary malignancy. In: Wanebo H, ed. Surgery for Gastrointestinal Cancer: A Multidisciplinary Approach. Philadelphia: LippincottRaven,1997:597–606.Google Scholar
- 11.Nakakuma K, Uemura K, Kono T, et al. Studies on anticancer treatment with anticancer drug injected into the ligated hepatic artery for liver cancer (preliminary report). Nichidoku Iho 1979; 24:675–682.Google Scholar
- 17.Cheng YF, Kan Z, Chen CL, et al. The efficacy and safety of preoperative lobar or segmental ablation via transarterial administration of ethiodol and ethanol mixture in the treatment of hepatocellular carcinoma: a clinical study. World J Surg 2000, in press.Google Scholar
- 18.Lin SY, Wu WH. Physical parameters and release behaviors of w/o/w multiple emulsions containing cosurfactants and different specific gravity of oil. Pharm Acta Helv 1991;66:342–347.Google Scholar
- 20.Evans CW. The invasion and metastatic behaviour of malignant cells. In: Evans CW, ed. The Metastatic Cell: Behavior and Biochemistry. London: Chapman & Hall, 1991:137–214.Google Scholar
- 22.Rost FD. Principles of fluorescence microscopy. Fluorochrometry. In: Rost FD, ed. Fluorescence Microscopy. Cambridge: Cambridge University Press, 1992;1–10:80–107.Google Scholar