Spectroscopy: Analysis of Structure

  • Peter R. Bergethon

Abstract

We have constructed a view of biochemical state space based predominantly on the ideas of a limited set of mechanical motions and the existence of electrical charge. Using just these basic concepts, we have been able to develop useful descriptions of light and electromagnetic radiation and subsequently of atomic and molecular structure. These ideas are developed from the same core physical concepts. Therefore, it is not surprising that we will find a substantial interaction between electromagnetic radiation and matter. A great deal of structural information can be gained by studying these interactions. In general we can explore the structure of biological molecules by focusing on either the wavelike nature or the quantum treatment of light. The first forms the basis of light-scattering (e.g., X-ray diffraction) experimental methods, and the second, the basis of spectroscopy. We take up spectroscopy next.

Keywords

Excited State Electron Paramagnetic Resonance Spectral Line Triplet State Internuclear Distance 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Further Reading

History

  1. Thomas N. C. (1991) The early history of spectroscopy. J. Chem. Ed., 74: 65–67.Google Scholar

General

  1. Kettle S. F. A., and Norrby L. J. (1990) The Brillouin zone: An interface between spectroscopy and crystallography. J. Chem. Ed., 67:1022–28. This explores an intersection of a subject from the different perspectives of a crystallographer and a spectroscopist. It is a nice demonstration of how the same natural system maps differently into two abstract spaces.Google Scholar
  2. Lykos P. (1992) The Beer-Lambert law revisited. J. Chem. Ed., 69: 730–32.CrossRefGoogle Scholar
  3. Macomber R. S. (1997) A unifying approach to absorption spectroscopy at the undergraduate level. J. Chem. Ed., 74: 159–82.CrossRefGoogle Scholar
  4. Stryer L. (1987) The molecules of visual excitation. Sci. Am., 257 (1): 42–50.PubMedCrossRefGoogle Scholar

Fluorescent Spectroscopy

  1. Stryer L. (1978) Fluorescence energy transfer as a spectroscopic rule. Ann. Rev. Biochem., 47: 819–46.PubMedCrossRefGoogle Scholar
  2. Stryer L. and Haugland R. P. (1967) Molecular rulers. Proc. Nat. Acad. Sci. USA, 58:719–26. The original application of Förster energy transfer to the purpose of intermolecular measuring.Google Scholar

Scattering Techniques for Evaluation of Macromolecules

  1. Cantor C. R. and Schimmel P. R. (1980) Biophysical Chemistry, vol. II, W. H. Freeman, New York.Google Scholar
  2. Freifelder D. M. (1982) Physical Biochemistry: Applications to Biochemistry and Molecular Biology, 2d ed. W. H. Freeman, New York.Google Scholar
  3. van Holde K. E. (1985) Physical Biochemistry, 2d ed. Academic Press, New York.Google Scholar

Raman Spectroscopy

  1. McCreery R. L. (1996) Analytical Raman spectroscopy: An emerging technology for practical application. American Laboratory, Feb:34X–34JJ.Google Scholar
  2. Pelletier M., and Davis K. (1996) Raman spectroscopy: The next generation. American Laboratory, Feb: 34C - 34N.Google Scholar

NMR Studies of Biological Application

  1. Sanders J. K. M., and Hunter B. K. (1993) Modern NMR Spectroscopy: A Guide for Chemists, 2d ed. Oxford University Press. Covers the principles for understanding conformational studies with NMR.Google Scholar
  2. Bryant R. G. (1996) The dynamics of water-protein interactions. Annu. Rev. Biophys. Biomol. Struct., 25: 29–53.PubMedCrossRefGoogle Scholar
  3. Dyson H. J., and Wright P. E. (1996) Insights into protein folding from NMR. Annu. Rev. Phys. Chem., 47: 369–95.PubMedCrossRefGoogle Scholar

Magnetic Resonance Imaging and Spectroscopy

  1. Edelman, R. R., and Warach, S. (1993) Magnetic resonance imaging. N. Engl. J. Med., 328: 708–16.PubMedCrossRefGoogle Scholar
  2. Oldendorf, W. and Oldendorf, W. Jr. (1991) MRI Primer. New York: Raven Press.Google Scholar
  3. Raichle M. E. (1994) Visualizing the mind. Sci. Am., 270 (4): 58–64.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Peter R. Bergethon
    • 1
  1. 1.Department of BiochemistryBoston University School of MedicineBostonUSA

Personalised recommendations