Preparation of Tissue-Cultured Material for Electron Microscopical Observation

  • Richard M. Devon

Abstract

Electron microscopy is an invaluable tool that has enabled investigators to visualize, at high resolution, the fine structure of cells. Many steps are required in preparing tissue for electron microscopy; these include fixation, dehydration, embedding (infiltration and polymerization), mounting, and sectioning, as well as collection and staining of the sections. At each step, it is possible to introduce artifacts that affect the end results. Therefore, an understanding of the rationale for performing each step (i.e., rates of penetration for various types of fixatives, buffer combinations, infiltration rates, and temperatures used) will assist the investigator in preventing, or at least reducing, the number of possible artifacts that can cause the deterioration of the final image obtained by the electron microscope.

Keywords

Toluidine Blue Osmium Tetroxide Potassium Ferricyanide Sodium Cacodylate Succinic Anhydride 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Further Reading

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Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Richard M. Devon

There are no affiliations available

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