Colony Cultures

  • Sergey Fedoroff
  • Arleen Richardson

Abstract

Colony cultures are based on the assumption that single viable cells can attach to the substratum, divide, and form a progeny of cells that constitute a cell colony. Colony cultures can be initiated either from the original disaggregated cell suspension made directly from animal tissue, or from primary or secondary cultures. When large numbers of viable cells are plated/cm2 of substratum, on culturing, a confluent layer of cells forms. When a small number of cells are plated/cm2 of substratum, then single, discrete cell colonies form. Colonies, especially ones initiated directly from tissues, are not necessarily uniform. The morphology and size of the colony depends on the kinds of cells plated, the degree of their differentiation, the cell generation time, the composition of the medium, the type of substratum, and the physical conditions. In practice, disaggregated tissue yields a variety of cells of varying degrees of maturity, resulting in heterogeneity in colony size and morphology. Colony cultures from secondary cultures usually have more uniform colonies.

Keywords

Glial Fibrillary Acidic Protein Culture Vessel Newborn Mouse Plating Efficiency Sodium Metasilicate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Further Reading

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Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Sergey Fedoroff
  • Arleen Richardson

There are no affiliations available

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