Outgrowth Assays and Cortical Slice Cultures

  • Valérie Castellani
  • Jürgen Bolz

Abstract

A simple outgrowth assay is described that allows the quantification of the growth behavior of specific axon populations on known and unknown substrates. Small explants prepared from various regions of the brain are placed on substrate-coated coverslips in Petriperm dishes with culture medium. This permits the observation of axonal growth patterns (axon elongation and branch formation) continuously over time. In addition, by blocking molecules that might control the growth and guidance of axons (e.g., with antibodies or lectins), this assay is well-suited to examine factors that promote or inhibit axonal growth and/or induce or prevent the formation of axon collaterals (Gotz et al., 1992; Henke-Fahle et al., 1996).

Keywords

Lateral Geniculate Nucleus Outgrowth Assay Slice Culture Cortical Slice Membrane Solution 
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Further Reading

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Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Valérie Castellani
  • Jürgen Bolz

There are no affiliations available

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