Outgrowth Assays and Cortical Slice Cultures
A simple outgrowth assay is described that allows the quantification of the growth behavior of specific axon populations on known and unknown substrates. Small explants prepared from various regions of the brain are placed on substrate-coated coverslips in Petriperm dishes with culture medium. This permits the observation of axonal growth patterns (axon elongation and branch formation) continuously over time. In addition, by blocking molecules that might control the growth and guidance of axons (e.g., with antibodies or lectins), this assay is well-suited to examine factors that promote or inhibit axonal growth and/or induce or prevent the formation of axon collaterals (Gotz et al., 1992; Henke-Fahle et al., 1996).
KeywordsLateral Geniculate Nucleus Outgrowth Assay Slice Culture Cortical Slice Membrane Solution
Unable to display preview. Download preview PDF.
- Götz, M., Novak, N., Bastmeyer, M., and Bolz, J. (1992), Membrane bound molecules in rat cerebral cortex regulate thalamic innervation. Development 116, 507–519.Google Scholar