Affinity Chromatography for Recombinant Proteins

  • Nathaniel G. Hentz
  • Vesna Vukasinovic
  • Sergi Díez
  • Manuel Valiente
  • Sylvia Daunert
Chapter

Abstract

The emergence of biotechnology has provided unique opportunities for the design and high level production of a variety of biomolecules, including recombinant proteins and peptides. Recombinant proteins/peptides with potential therapeutic value are being rapidly developed by the pharmaceutical industry. The purification of these therapeutic proteins must be performed under the most stringent conditions in order to achieve the homogeneity, purity and potency required by Federal regulations. Thus, comes the need for expanding the repertoire of conventional bioseparation techniques with novel methods of purification.

Keywords

Fusion Protein Recombinant Protein Target Protein Affinity Chromatography Affinity Matrix 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • Nathaniel G. Hentz
    • 1
  • Vesna Vukasinovic
    • 1
  • Sergi Díez
    • 2
  • Manuel Valiente
    • 2
  • Sylvia Daunert
    • 1
  1. 1.Department of Chemistry and Center of Membrane SciencesUniversity of KentuckyLexingtonUSA
  2. 2.Departament de Química Unitat de Química AnalíticaUniversitat Autònoma de BarcelonaBellaterra (Barcelona)Spain

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