The overall goal of this work was to prepare synthetic receptor matrices that combined the Properties of antibodies (specificity and selectivity of recognition) with those of synthetic polymeric membranes (stability, durability, solvent resistance, etc.). The approach taken involved imprinting molecular recognition complexes onto membrane pore surfaces using photochemistry. Fluor-containing membranes possessing photo-imprinted surfaces can be used in competitive binding scintillation proximity assays. This would overcome the two major limitations of radioimmunoassay, i.e., antibodies would be replaced by stable synthetic receptors, and the bound radioligand would not need to be separated from the unbound radioligand because only the bound radioligand would be in close enough proximity to the fluors in the membrane to induce scintillations. Furthermore, this would permit detection of beta-emitting radioligands without using a scintillation cocktail. This would have a positive economic and environmental impact by greatly reducing the generation of “mixed” (radioactive and organic) waste, the disposal of which is difficult and expensive.
KeywordsMolecular Imprint Polymer Molecular Recognition Methacrylic Acid Imprint Polymer Template Molecule
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