Methodology for the Extraction, Purification, and Determination of Plant Growth Substances
Plant growth substances are involved in nearly all aspects of the plant’s life cycle as deduced from the fact that exogenous applications by them profoundly affect plant responses. However, it is difficult to detect plant growth substances within the plant for numerous reasons. At the present time it is not known whether plant growth substances act at certain levels in a dose-dependent manner or if they remain constant, while changes in tissue sensitivity (receptors) are more closely related to a given response. Our knowledge in the area of plant growth substances is still quite limited and the major reason is that the techniques for their analysis are still very cumbersome. There are many methods for extracting, purifying, and quantifying plant growth substances; however, it is not the intent in this section to provide an exhaustive review of the literature in the area. This chapter is intended to be a basic outline of all steps from tissue preparation through final identification and quantification of plant growth substances. However, for detailed protocols see Rivier and Crozier (1987) and Hedden (1993).
KeywordsAmmonium Sulphate Polyclonal Antiserum Plant Growth Substance Solvent Partitioning Immobilize Antigen
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