Characterization of Receptors for Insulin and Insulin-Like Growth Factor-1 on FRTL-5 Thyroid Cells

  • N. Perrotti
  • C. M. Rotella
  • F. V. Alvarez
  • L. D. Kohn
  • S. Taylor
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 261)

Abstract

The development of the FRTL and FRTL-5 thyroid cells (Fig. 1A) by F.S. Ambesi-Impiombato and his colleagues (1,2) moved research in the thyroid area from a concern with the thyrotropin receptor to an examination of receptor interactions or cross-talk. These cells require TSH for their growth (Fig. 1B). However, the TSH action requires the presence of insulin and serum factors, one of which we now know to be IGF-I (Fig. 1B); no single factor, TSH, insulin, or IGF-I, can reproduce the mixture of all. Further it is evident that the insulin and IGF-I actions are not identical; thus when concentrations of insulin as high as 10 micrograms per ml are present, the addition of only 50 or 100 ng/ml of IGF-I is still significantly additive to TSH and insulin in stimulating an increase in cell number.

Keywords

Insulin Receptor Thyroid Cell Insulin Binding Scatchard Plot Normal Human Serum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • N. Perrotti
    • 1
  • C. M. Rotella
    • 1
  • F. V. Alvarez
    • 1
  • L. D. Kohn
    • 1
  • S. Taylor
    • 1
  1. 1.Diabetes Branch NIDDKNational Institutes of HealthBethesdaUSA

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