Electron Microscopic Preembedding Double-Immunostaining Methods

  • Csaba Leranth
  • Virginia M. Pickel

Abstract

During the last decade, immunocytochemistry has become one of the major tools for the light and electron microscopic identification of neurons containing classical transmitters and neuropeptides (e.g., Cuello, 1983; Polak and Van Noorden, 1983; Steinbush et al., 1978). The immunocytochemical studies have provided a complete characterization of the regional distributions and ultrastructural morphology of a variety of chemically specific neurons (Elde etat., 1976; Pickel, 1981). More recently, dual immunocytochemical labeling of two antigens in the same section prepared for electron microscopy has permitted the characterization of transmitters within pre- and post synaptic junctions in the central nervous system (Leranth et al., 1984; van den Pol, 1985a; Milner et al., 1987).

Keywords

Tyrosine Hydroxylase Colloidal Gold Glutamic Acid Decarboxylase Primary Antiserum Double IMMUNOSTAINING 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Csaba Leranth
    • 1
  • Virginia M. Pickel
    • 2
  1. 1.Section of Neuroanatomy and Department of Obstetrics and GynecologyYale University School of MedicineConnecticutUSA
  2. 2.Department of Neurology and Neuroscience, Laboratory of NeurobiologyCornell University Medical CollegeNew YorkUSA

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