Processing and Analysis of Neuroanatomical Images

  • Michael T. Shipley
  • Jesus Luna
  • John H. McLean

Abstract

Today, neuroanatomists have an almost bewildering array of techniques that are being used to study the organization of the nervous system along dimensions that were not even conceived 10 to 15 years ago. Brain circuits are no longer viewed as wiring diagrams interlinking classical brain structures but rather as subpopulations of neurons differing along morphological and neurochemical lines having interconnections with other equally specific subpopulations of neurons in multiple other brain structures. No longer are neural circuits “excitatory” or “inhibitory.” Neurons may contain several transmitters/modulators, and the actions of modulators may depend on the moment-to-moment status of their target cells. The levels of these neuroac-tive molecules may vary with the animal’s functional, humoral, or developmental state. On the postsynaptic side, multiple receptor subtypes are well established for many transmitter systems; these link presynaptic inputs to a growing multitude of channel types and second messenger systems, which may or may not have direct actions on the genome or gene products of the target cell. Methods for studying these phenomena in brain sections are now available, and there are exciting possibilities to apply similar techniques to in vitro brain-slice preparations to study functioning neuroanatomy.

Keywords

Video Camera Olfactory Bulb Gray Level Video Image Gray Image 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Allen, R. D., Travis, J. L., Allen, N. S., and Yilmaz, H., 1981a, Video-enhanced contrast polarization (AVEC-POL) microscopy: A new method applied to the detection of birefringence in the motile reticulopodial network of Allogromia laticollaris, Cell Motu. 1:275–289.CrossRefGoogle Scholar
  2. Allen, R. D., Allen, N. S., and Travis, J. L., 1981b, Video-enhanced contrast, differential interference contrast (AVEC-DIC) microscopy: A new method capable of analyzing microtu-bule-related motility in the reticulopodial network of Allogromia laticollaris, Cell Motu. 1:291–302.CrossRefGoogle Scholar
  3. Blaha, G., Blair, W., Nickell, W. T., and Shipley, M. T., 1984, Cholinergic (CH) receptors in the rat olfactory bulb: Nicotinic (N) and muscarinic (M) cholinergic receptors are segregated and coincide with acetylcholinesterase (AChE), Neurosci. Soc. 10:1183.Google Scholar
  4. Glaser, E. M, Tagamets, M., McMullen, N. T., and Van der Loos, H., 1983, The image-combing computer microscope—an interactive instrument for morphometry of the nervous system, J. Neurosci. Methods 8:17–32.PubMedCrossRefGoogle Scholar
  5. Inoue, S., 1986, Video Microscopy, Plenum Press, New York.Google Scholar
  6. Kauer, J. S., 1988, Real-time imaging of evoked activity in local circuits of the salamander olfactory bulb, Nature 331:166–168.PubMedCrossRefGoogle Scholar
  7. McLean, J. H., and Shipley, M. T., 1987a, Serotonergic afferents to the rat olfactory bulb: I. Origins and laminar specificity of serotonergic inputs in the adult rat, J. Neurosci. 7:3016–3028.PubMedGoogle Scholar
  8. McLean, J. H., and Shipley, M. T., 1987b, Serotongeric afferents to the rat olfactory bulb: II. Changes in fiber distribution during development, J. Neurosci. 7:3029–3039.PubMedGoogle Scholar
  9. McLean, J. H., Nickell, W. T., and Shipley, M. T., 1986, Afferent connections to the horizontal limb of diagonal band, Neurosci. Soc. 12:351.Google Scholar
  10. McLean, J. H., Shipley, M. T., Nickell, W. T., Aston-Jones, G., and Reyher, C. K. H., 1988, Chemoanatomical organization of the noradrenergic input from locus coeruleus to the olfactory bulb of the adult rat, J. Comp. Neurol, (in press).Google Scholar
  11. Nickell, W. T., and Shipley, M. T., 1988, Two anatomically specific classes of candidate choli-noceptive neurons in the rat olfactory bulb, J. Neurosci. 8:4482–4491.PubMedGoogle Scholar
  12. Shipley, M. T., McLean, J. H., and Behbehani, M. M., 1987, Heterogeneous distribution of neurotensin-like immunoreactive neurons and fibers in the midbrain periaqueductal gray of the rat, J. Neurosci. 7:2025–2034.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Michael T. Shipley
    • 1
  • Jesus Luna
    • 2
  • John H. McLean
    • 2
  1. 1.Department of Anatomy and Cell Biology, Division of Neurobiology, and Department of NeurosurgeryUniversity of Cincinnati College of MedicineCincinnatiUSA
  2. 2.Department of Anatomy and Cell Biology, Division of NeurobiologyUniversity of Cincinnati College of MedicineCincinnatiUSA

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