In Vitro Studies on Docosahexaenoic Acid in Human Platelets
Arachidonic acid (5,8,11,14–20:4 or 20:4w6) is the main polyunsaturated fatty acid of blood and vascular cells and it has been recognized as the precursor of numerous oxygenated derivatives exhibiting a variety of biological activities (1 – 3). In blood platelets, 20:4w6 is almost exclusively located in glycerophospholipids, from where it is fastly mobilized upon cell activation by aggregating agents like thrombin, collagen or the calcium ionophore A 23187 (4–6). Such a mobilization is the limiting step of prostaglandin endoperoxides and thromboxane A2 formation, the potent proaggregatory molecules, as well as of that of 12-lipoxygenase products.
KeywordsDocosahexaenoic Acid Human Platelet Prostaglandin Endoperoxide Lipoxygenase Product Polyenoic Acid
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- 4.Smith, J.B. The prostanoids in hemostasis and thrombosis. Am. J. Pathol. 99: 742 (1980).Google Scholar
- 6.Lagarde, M. Roles of cyclooxygenase and lipoxygenase metabolites in platelets. In. “Platelets in Biology and Pathology III”, D.E. Mc Intyre, and J.L Gordon, eds. Elsevier, Amsterdam p.269 ( 1987 ).Google Scholar
- 12.Croset, M. and Lagarde, M. In vitro incorporation and metabolism of icosapentaenoic and docosahexaenoic acids in human platelets. Effect on aggregation. Thromb. Haemostas. 56:57 (1986).Google Scholar
- 13.Croset, M., Guichardant, M. and Lagarde, M. Different metabolic behavior of long chain n-3 polyunsaturated fatty acids in human platelets. Biochim. Biophys. Acta 1988, In press.Google Scholar
- 14.Carey, F., Menashi,S. and Crawford, N. Localization of cyclooxygenase and thromboxane synthetase in human platelet intracellular membranes. Biochem. J. 204: 847 (1982).Google Scholar
- 17.Hajarine, M. and Lagarde, M. Liberation and oxygenation of polyenoic acids in stimulated platelets. Biochimie 1988. in press.Google Scholar
- 21.Galloway, J.H., Cartwright, I.J., Woodcoek, B.E., Greaves,M., Russel, G.G. and Preston, F.E. Effects of dietary fish oil supplementation on the fatty acid composition of the human platelet membrane: demonstration of selectivity in the incorporation of eicosapentaenoic acid into membrane phospholipid pools. Clin. Sci. 68: 449 (1985).Google Scholar