Polyunsaturated Fatty Acid Metabolism in Endothelial Cells: Relationship to Eicosanoid Formation
Arachidonic acid, a member of the omega-6 (n−6) class of polyunsaturated fatty acids, plays a very important role in endothelial function. This is illustrated in Fig. 1. When the endothelium is stimulated by agonists such as thrombin or histamine, arachidonic acid (20:4) is hydrolyzed from phospholipids and converted to several vasoactive compounds. One of the major products is prostacyclin (PGI2), a potent platelet anti-aggregatory agent and vasodilator1. Many other prostaglandin (PG) and lipoxygenase (LX) products are produced by various kinds of endothelial cells2, among the major ones being prostaglandin E2 (PGE2) and 15-hydroxyeicosatetraenoic acid (15-HETE).
KeywordsHigh Performance Liquid Chromatography Arachidonic Acid Human Umbilical Vein Endothelial Cell Eicosapentaenoic Acid Human Endothelial Cell
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