Nephrotoxicity pp 337-342 | Cite as

Alpha-Methylglucose Uptake by Isolated rat Kidney Proximal Tubular Cells as a Parameter for Cell Integrity in Vitro

  • P. J. Boogaard
  • G. J. Mulder
  • J. F. Nagelkerke

Abstract

A large number of nephrotoxins cause damage to the proximal tubule in vivo. Many typical in vivo tubular cell functions are conserved in freshly isolated rat kidney cells (Ormstad et al., 1981; Jones et al., 1979). An advantage of the use of isolated cells as compared to the in vivo experiment is that it permits a defined quantitatively and qualitatively cellular environment, which allows study of the relationship between the concentration of a nephrotoxin, exposure time and effect. Extra-renal effects can also be excluded, thus isolated tubular cells are very suitable to study the effects of nephrotoxins which exert their effect directly at the tubular site.

Keywords

Tubular Cell Proximal Tubular Cell Isopycnic Centrifugation Kidney Tubular Cell High Density Medium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • P. J. Boogaard
    • 1
  • G. J. Mulder
    • 1
  • J. F. Nagelkerke
    • 1
  1. 1.Division of Toxicology, Center for Bio-Pharmaceutical SciencesUniversity of LeidenLeidenThe Netherlands

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