Alpha-Methylglucose Uptake by Isolated rat Kidney Proximal Tubular Cells as a Parameter for Cell Integrity in Vitro
A large number of nephrotoxins cause damage to the proximal tubule in vivo. Many typical in vivo tubular cell functions are conserved in freshly isolated rat kidney cells (Ormstad et al., 1981; Jones et al., 1979). An advantage of the use of isolated cells as compared to the in vivo experiment is that it permits a defined quantitatively and qualitatively cellular environment, which allows study of the relationship between the concentration of a nephrotoxin, exposure time and effect. Extra-renal effects can also be excluded, thus isolated tubular cells are very suitable to study the effects of nephrotoxins which exert their effect directly at the tubular site.
KeywordsTubular Cell Proximal Tubular Cell Isopycnic Centrifugation Kidney Tubular Cell High Density Medium
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