In Vitro Perfusion of Human Placenta

A Workshop Report
  • Henning Schneider
  • Joseph Dancis
Part of the Trophoblast Research book series (TR)


The workshop on in vitro perfusion of human placenta concentrated on recent advances that might influence the use of the techniques. Presentations were made by Henning Schneider, Nicholas Illsley, and Richard Miller. Maurice Panigel and Heinz-Peter Leichtweiss introduced the discussion The allotted time passed quickly, with questions and conversations overflowing into the corridors during the rest of the conference. In the following, we shall review briefly the workshop and attempt to summarize the ensuing conversations in a form that we hope will be useful to the reader.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bissonnette, J.M. (1981) Studies in vivo on glucose transfer across the guinea pig placenta. Placenta Suppl. 2, 155–162.CrossRefGoogle Scholar
  2. Bloxam, D.L. and Bobinski, P.M. (1984) Energy metabolism and glycolysis in the human placenta during ischemia and in normal labor. Placenta 5, 381–394.PubMedCrossRefGoogle Scholar
  3. Dancis, J., Jansen, V., Kayden, H.J., Schneider, H., and Levitz, M. (1973) Transfer across perfused human placenta. II. Free fatty acids. Pediatr. Res. 7, 192–197.PubMedCrossRefGoogle Scholar
  4. Duft, R. and Schneider, H. (1985) Ischemic postpartum changes in human placental tissue. Unpublished observations.Google Scholar
  5. Eaton, B.M. and Yudilevich, D.L. (1981) Uptake and asymmetric efflux of amino acids at maternal and fetal sides of placenta. Am. J. Physiol. 241, C106 - C112.Google Scholar
  6. Eaton, B.M. and Contractor, S.F. (1985) Maternal to fetal movement of creatinine as a measure of perfusion efficiency and diffusional transfer in the isolated human placental lobule. Placenta 6, 341–346.PubMedCrossRefGoogle Scholar
  7. Harkness, R.A., Coade, S.B., Simmonds, R.J., and Duffy, S. (1985) Effect of a failure of energy supply on adenine nucleotide breakdown in placentae and other fetal tissues from rat and guinea pig. Placenta 6, 199–216.PubMedCrossRefGoogle Scholar
  8. Halliwell, B. (1978) Biochemical mechanisms accounting for the toxic action of oxygen on living organisms: the key role of superoxide dismutase. Cell Biol. Int. Rep. 2, 113–128.PubMedCrossRefGoogle Scholar
  9. Hedley, R. and Bradbury, M.W.B. (1980) Transport of polar non-electrolytes across the intact and perfused guinea pig-placenta. Placenta 1, 227–285.CrossRefGoogle Scholar
  10. Illsley, N. (1985) A simplified method for measurement of unidirectional transplacental flux. Unpublished observation.Google Scholar
  11. Illsley, N.P., Hall, S., and Stacey, T.E. (1987) The modulation of glucose and oxygen transfer across the human placenta by intervillous flow rates: an in vitro perfusion study. Trophoblast Res. 2, 537–546.Google Scholar
  12. Kelman, B.J. and Sasser, L.B. (1977) Methylmercury movements across the perfused guinea pig placenta in late gestation. Toxicol. Appl. Pharmacol. 39, 119–127.PubMedCrossRefGoogle Scholar
  13. Maguire, M.H., Westermeyer, F.A., and King, Ch.R. (1985) Adenosine, inosine and hypoxanthine levels in human term placenta. Abst. 10th Rochester Trophoblast Conference, 34.Google Scholar
  14. Miller, R.K., Wier, P.J., Maulik, D., and di Sant’Agnese, P.A. (1985) Human placenta in vitro: Characterization during 12 hr of dual perfusion. Contrib. Gyn. Obstet. 13, 77–84.Google Scholar
  15. Penfold, P., Drury, L., Simmonds, R.J., and Hytten, F.E. (1981) Studies of a single placental cotyledon in vitro: I. The preparation and its viability. Placenta 2, 149–154.PubMedCrossRefGoogle Scholar
  16. Schneider H. (1983) Placental transfer of D- and L-glucose studies in the in vitro perfused lobule-using bolus injections. Unpublished.Google Scholar
  17. Schneider, H., Pangiel, M., and Dancis, J. (1972) Transfer across the perfused human placenta of antipyrine, sodium and leucine. Am. J. Obstet. Gynecol. 114, 82 2828.Google Scholar
  18. Schneider, H., Proegler, M., and Duft, R. (1986) Transfer of antipyrine, 3H2O, L-glucose and a-aminoisobutyric acid across the in vitro perfused human placental lobe. Trophoblast Res. 2, 481–489.Google Scholar
  19. Schneider, H., Proegler, M., and Sodha, R.J. (1985) Effect of flow rate ratio on the diffusion of antipyrine and 3H2O in the isolated dually in vitro perfused lobe of human placenta. Contrib. Gyn. Obstet. 13, 114–123.Google Scholar
  20. Schroeder, H., Leichtweiss, H.-P., and Rachor, D. (1985) Passive exchange and the distribution of flows in the isolated human placenta. Contrib. Gyn. Obstet. 13, 106–113.Google Scholar
  21. Sodha, R.J., Proegler, M., and Schneider, H., (1984) Transfer and metabolism of norepinephrine studied from maternal-to-fetal and fetal-to-maternal sides in the in vitro perfused human placenta lobe. Am. J. Obstet. Gynecol. 148, 474481.Google Scholar
  22. Wier, P.J. and Miller, R.K. (1985) Oxygen transfer as an indicator of perfusion variability in the isolated human placental lobule. Contrib. Gyn. Obstet. 13, 127–131.Google Scholar
  23. Wooton, R. Illsley, N., and Hall, S., (1985) A new method for measuring unidirectional transplacental flux. Clin. Phys. Physiol. Meas. 6, 47–57.Google Scholar
  24. Yudilevich, D.L., Eaton, B.M., Short, A.H., and Leichtweiss, H-P., (1979) Glucose carriers at maternal and fetal sides of the trophoblast in guinea pig placenta. Am. J. Physiol. 237, C205 - C212.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1987

Authors and Affiliations

  • Henning Schneider
    • 1
  • Joseph Dancis
    • 2
  1. 1.Division of Perinatal Physiology Department of ObstretricsUniversity of ZuerichZuerichSwitzerland
  2. 2.Department of Pediatrics, School of MedicineNew York UniversityNew YorkUSA

Personalised recommendations