Transfer of Antipyrine, H2O, L-Glucose and α-Aminoisobutyric Acid Across the in Vitro Perfused Human Placental Lobe

  • Henning Schneider
  • Mariette Proegler
  • Reto Duft
Part of the Trophoblast Research book series (TR)


The dual circuit in vitro perfusion of an isolated lobe of human placenta is a useful experimental technique to study transfer of material across the placenta and to identify factors influencing exchange between maternal and fetal circulation. It has repeatedly been noted that there is considerable variation in results among experiments which are not fully explained by differences in size of the perfused lobe (Schneider et al., 1972; Welsch, 1980). While flow rates in the two perfusion circuits can easily be controlled, the surface area of exchange, shunts, and degree of overlap of the two circulations remain major factors causing interexperimental variability. Antipyrine which rapidly equilibrates across most biological membranes has been proposed as a reference for normalization of experimental data on transfer (Dancis et al., 1973). After this correction parameters like membrane permeability or specific transport mechanisms can be explored. This concept should be valid for the study of test compounds which are similarly affected in transfer by the uncontrollable variables as is antipyrine.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bissonnette, J.M., Cronau, J.Z., Richards, L.L., and Wickham, W.K. (1979) Placental transfer of water and nonelectrolytes during a single circulatory passage. Am. J. Physiol. 236 (1), 47–52.Google Scholar
  2. Bissonnette, J.M. (1981) Studies in vivo of glucose transfer across the guinea pig placenta. Placenta Suppl. 2, 155–162.CrossRefGoogle Scholar
  3. Brodie, B.B., Axelrod, J., Soberman, R., and Levy, B.B. (1949) The estimation of antipyrine in biological materials. J. Biol. Chem. 179, 25–29.PubMedGoogle Scholar
  4. Challier, J.C., D’Athis, P., Guerre-Millo, M., and Nandakumaran, M. (1983) Flow-dependent transfer of antipyrine in the human placenta in vitro. Reprod. Nutr. Develop. 23, 41–50.CrossRefGoogle Scholar
  5. Dancis, J., Jansen, V., Kayden, H.J., Schneider, H., and Levitz, M. (1973) Transfer across perfused human placenta. II. Fatty acids. Ped. Res. 7, 192–197.CrossRefGoogle Scholar
  6. Hedley, R. and Bradbury, M.W.B. (1980) Transport of polar nonelectrolytes across the intact and perfused guinea pig placenta. Placenta 1, 277–285.PubMedCrossRefGoogle Scholar
  7. Kelman, B.J. and Sikov, M.R. (1983) Clearance of a-aminoisobutyric acid during in situ perfusion of the guinea pig placenta. Trophoblast Res. 1, 71–80.Google Scholar
  8. Lassen, N.A. and Perl, W. (1979) Tracer Methods in Medical Physiology. Raven Press, New York.Google Scholar
  9. Meschia, G., Battaglia, F.C., and Bruns, P.D. (1967) Theoretical and experimental study of transplacental diffusion. J. Appl. Physiol. 22, 1171–1178.PubMedGoogle Scholar
  10. Schneider, H., Panigel, M., and Dancis, J. (1972) Transfer across the perfused human placenta of antipyrine, sodium and leucine. Am. J. Obstet. Gynecol. 114, 822828.Google Scholar
  11. Schneider, H. and Dancis, J. (1974) Amino acid transport in human placental slices. Am. J. Obstet. Gynecol. 120, 1092–1098.PubMedGoogle Scholar
  12. Schneider, H., Moehlen, K.H., and Dancis, J. (1979) Transfer of amino acids across the in vitro perfused human placenta. Ped. Res. 13, 236–240.CrossRefGoogle Scholar
  13. Schneider, H. and Huch, A. (1985) Dual in vitro perfusion of an isolated lobe of human placenta: method and instrumentation. In: In Vitro Perfusion of Human Placental Tissue, (eds.), H. Schneider and J. Dancis, Basel, Karger, pp. 40–47.Google Scholar
  14. Schneider, H., Proegler, M., and Sodha, R.J. (1985) Effect of flow rate ratios on the diffusion of antipyrine and 3H2O in the isolated dually in vitro perfused lobe of the human placenta. In: In Vitro Profusion of Human Placental Tissues, (eds.), H. Schneider and F. Dancis, Basel, Karger, pp. 114–123.Google Scholar
  15. Sodha, R.J., Proegler, M., and Schneider, H. (1984) Transfer and metabolism of norepinephrine studied from maternal-to-fetal and fetal-to-maternal sides in the in vitro perfused human placental lobe. Am. J. Obstet. Gynecol. 148, 474484.Google Scholar
  16. van Dijk, J.P. and van Kreel, B.K. (1978) Transport and accumulation of aaminoisobutyric acid (A.I.B.) in the guinea pig placenta. Pflüegers Arch. 377, 217–224.CrossRefGoogle Scholar
  17. Welsch, F. (1980) Effects of acetylcholine on the clearance of 3H-antipyrine in bilaterally perfused lobules of human term placenta. Gynecol. Obstet. Invest. II, 49–55.CrossRefGoogle Scholar
  18. Yudilevich, D.L., Eaton, B.M., and Mann, G.E. (1981) Carriers and receptors at the maternal and fetal sides of the placenta studied by a single-circulation paired-tracer dilution technique. Placenta Suppl. 2, 139–149.Google Scholar

Copyright information

© Springer Science+Business Media New York 1987

Authors and Affiliations

  • Henning Schneider
    • 1
  • Mariette Proegler
    • 1
  • Reto Duft
    • 1
  1. 1.Division of Perinatal Physiology Department of ObstetricsUniversity of ZuerichZuerichSwitzerland

Personalised recommendations