Nucleotide Interconversion and Breakdown in the Dually Perfused Guinea Pig Placenta
Alterations in placental purine metabolism can occur due to diminished oxygen supply during labor (van Kreel and Wallenburg, 1980; Wallenburg and van Kreel, 1980; van Kreel and van Dijk, 1982). To study purine metabolism in the placenta without interference from other organs perfusion of the isolated placenta, or organ culture of trophoblastic cells can be used (Vettenranta and Raivo, 1984). The first objective of this study was to investigate nucleotide breakdown in placental tissue, and to quantitate the extraction of nucleosides and purines during perfusion under various experimental conditions of oxygen supplementation.
Unable to display preview. Download preview PDF.
- Bishop, C., Rankine, D.M., and Talbott, J.H. (1958) The nucleotides in normal human blood. J. Biol. Chem. 234, 1233–1237.Google Scholar
- Peeters, L.L. and Wallenburg, H.C.S. (1983) Technique for chronic blood sampling from the ovarian vein in the pregnant guinea pig. Bio. Res. Preg. Prenatal. 5, 188–120.Google Scholar
- van Kreel, B.K. and Wallenburg, H.C.S. (1980) Hypoxanthine metabolism and transfer in the pregnant Rhesus monkey. J. Develop. Physiol. 2, 365–372.Google Scholar
- van Kreel, B.K. (1985) The estimation of the apparent standard free energy change of a biochemical reaction from the standard free energy of formation and apparent free energy of ionization of the participating molecules and its application to the reactions of the purine metabolism. Biochem. Education 13 (3), 125–130.CrossRefGoogle Scholar