Trophoblast Cell Culture
Using procedures currently available it is possible to obtain viable trophoblasts from both early and term placenta. In accomplishing this it is important to use fresh placental tissue. Dispersion of cells from the primary tissue can be achieved with some preparations of crude collagenase or with some preparation of trypsin. There was wide agreement that one’s ultimate success in obtaining healthy trophoblast cells is dependent on choosing the proper lot of either protease. There was general agreement in the workshop that syncytium is lost during sequential trypsinization of placenta, probably by lysis, though one participant suggested that the syncytium may end up in the red blood cell pellet in a Percoll gradient. Calf serum should also be screened, since some lots are superior to others.
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