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Characterization of Isolated Cells in Primary Culture from Human term Placenta by Electron Microscopy and Immunohistochemistry

  • Juliet O. Lobo
  • Francis L. Bellino
  • Alan Siegel
Chapter
Part of the Trophoblast Research book series (TR)

Abstract

During pregnancy the syncytiotrophoblast layer of the human placenta elaborates both protein and steroid hormones (Gaspard et al., 1980; Simpson and MacDonald, 1981; Winkel et al., 1980). The mitotically inactive but biochemically functional syncytiotrophoblasts are formed in vivo by the fusion of the underlying mitotically active, mature, mononucleated cytotrophoblast cells (Boyd and Hamilton, 1967; Enders, 1965; Pierce and Midgley, 1963; Wislocki and Dempsey, 1955; Wynn, 1972).

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References

  1. Boyd, J.D., and Hamilton,W.J. (1967) Development and structure of the human placenta from the end of the third month of gestation. J. Obstet. Gynecol. of Brit. Commonwealth 74, 161–226.Google Scholar
  2. Carr, M.C. (1972) Study into the mechanisms of damage of human placental syncytiotrophoblast. ii. Histologic comparison of plasma-induced damage and specific enzyme-induced damage. Gynecol. Invest. 3, 203–214.Google Scholar
  3. Carter, J.E. (1964) Morphologic evidence of syncytial formation from the cytotrophoblastic cells. Obstet. Gynecol. 23, 647–656.Google Scholar
  4. Contractor, S.F. (1979) Receptors in cultured human trophoblast cells. In Protein Transmission Through Living Membranes, Hemmings, W.A. (ed.), Elsevier/North Holland Biomedical Press, Amsterdam, pp 3–75.Google Scholar
  5. Contractor, S.F., Routledge, A. and Sooranna, S.R. (1984) Identification and estimation of cell types in mixed primary cell cultures of early and term human placenta. Placenta 5, 41–54.Google Scholar
  6. Cotte, C., Easty, G.C., Neville, A.M., and Monaghan, P. (1980) Preparation of highly purified cytotrophoblast from human placenta with subsequent modulation to form syncytiotrophoblast in monolayer culture. In Vitro 16, 639–646.Google Scholar
  7. Deal, C.L., and Guyda, H.J. (1983) Insulin receptors of human term placental cells and choriocarcinoma (JEG-3) cells: characteristics and regulation. Endocrinol. 112, 1512–1523.Google Scholar
  8. de Ikonicoff, L.K. and Cedard, L. (1973) Localization of human chorionic gonadotropic and somatomammotropic hormones by the peroxidase immunohistoenzymologic method in villi and amniotic epithelium of human placentas (from six weeks to term). Am. J. Obstet. Gynecol. 116 1124–1132.Google Scholar
  9. Enders, A.C. (1965) Formation of syncytium from cytotrophoblast in the human placenta. Obstet. Gynecol. 25, 378–386.Google Scholar
  10. Foldes, J.J., and Schwartz, J. (1972) Some new aspects in human trophoblast cultures. Experientia 28,1468–1470.Google Scholar
  11. Fox, H., and Kharkongor, F.N. (1970a) Morphology and enzyme histochemistry of cells derived from placental villi in tissue culture. J. Path. 101 267–276.Google Scholar
  12. Fox, H.,and Kharkongor, F.N. (1970b) Immunofluorescent localization of chorionic gonadotrophin in the placenta and in tissue cultures of human trophoblast. J. Path. 101 277–282.Google Scholar
  13. Gaspard, U.J., Hustin, J., Reuter, A.M., Lambotte, R., and Franchimont, P. (1980) Immunofluorescent localization of placental lactogen, chorionic gonadotropin and its alpha and beta subunits in organ cultures of human placenta. Placenta 1, 135–144.Google Scholar
  14. Huot, R.I., Foidart, J.M. and Stromberg, K. (1979) Effects of culture conditions on the synthesis of human chorionic gonadotropin by placental organ cultures. In Vitro 15, 497–502.Google Scholar
  15. Kaspi, T. and Nebel, L. (1974) Isolation of syncytiotrophoblast from human term placentas. Obstet. and Gynaecol. 43, 549–557.Google Scholar
  16. Kawata, M., Parnes, J.R., and Herzenberg, L.A. (1984) Transcriptional control of HLA- A,B,C antigen in human placental cytotrophoblast isolated using trophoblast-and HLA-specific monoclonal antibodies and the fluorescence-activated cell sorter. J. Exp. Med. 160, 633–651.Google Scholar
  17. Kliman, H.J., Nestler, J.E., Sermasi, E., Sanger, J.M. and Strauss, J.F. III (1986) Purification, characterization and in vitro differentiation of cytotrophoblasts from human term placentae. Endocrinol. 118, 1567–1582.Google Scholar
  18. Kurman, R.J., Main, C.S., and Chen, H.C. (1984) Intermediate trophoblast: a distinctive form of trophoblast with specific morphological, biochemical and functional features. Placenta 5, 349–370.Google Scholar
  19. Lobo, J.O., Bellino, F.L., and Bankert, L. (1985) Estrogen synthetase activity in human term placental cells in monolayer culture. Endocrinol. 116, 889–895.Google Scholar
  20. Loke, Y.W. (1983) Human trophoblast in culture. In Biology of Trophoblast, Loke, Y.W. and Whyte, A. (eds.), Elsevier Science Publishers, Amsterdam, pp 663701.Google Scholar
  21. Loke, Y.W., and Borland, R. (1970) Immunofluorescent localization of chorionic gonadotropin in monolayer cultures of human trophoblast cells. Nature 228, 561–562.Google Scholar
  22. Loke, Y.W., Wilson, D.V. and Borland, R. (1972) Localization of human chorionic gonadotropin in monolayer cultures of trophoblast cells by mixed agglutination. Am. J. Obstet. Gynecol. 113, 875–879.Google Scholar
  23. Martinez, F., Cheung, S.W., Crane, J.P. and Arias, F. (1983) Use of trophoblast cells in tissue culture for fetal chromosomal studies. Am. J. Obstet. Gynecal. 147, 542–547.Google Scholar
  24. Nelson, D.M., Meister, R.K., Ortman-Nabi, J., Sparks, S. and Stevens, V.C. (1986) Differentiation and secretory activities of cultured human placental cytotrophoblasts. Placenta 7, 1–6.Google Scholar
  25. Pierce, G.B. and Midgley, A.R. (1963) The origin and function of human syncytiotrophoblastic giant cells. Am. J. Pathol. 43, 153–172.Google Scholar
  26. Simpson, E.R. and MacDonald, P.C. (1981) Endocrine physiology of the placenta. Ann. Rev. Physiol. 43, 163–188.Google Scholar
  27. Stromberg, K., Azizkhan, J.C. and Speeg, K.V., Jr. (1978) Isolation of functional human trophoblast cells and their partial characterization in primary culture. In Vitro 14, 631–638.Google Scholar
  28. Jogee, M., Myatt, L., Moore, P. and Elder, M.G. (1983) Prostacyclin production by human placental cells in short term culture. Placenta 4, 219–229.Google Scholar
  29. Thiede, H.A. (1960) Studies of the human trophoblast in tissue culture. I. Cultural methods and histochemical staining. Am. J. Obstet. Gynecol. 79, 636–647.Google Scholar
  30. Thiede, H.A., and Choate, J.W. (1963) Chorionic gonadotropin localization in the human placenta by immunofluorescent staining. II. Demonstration of hCG in the trophoblast and the amnion epithelium of immature and mature placentas. Obstet. Gynecol. 22, 433–443.Google Scholar
  31. Winkel, C.A., Snyder, J.M., MacDonald, P.C. and Simpson, E.R. (1980) Regulation of cholesterol and progesterone synthesis in human placental cells in culture by serum lipoproteins. Endocrinol. 106,1054–1060.Google Scholar
  32. Wislocki, G.B. and Dempsey, E.W. (1955) Electron microscopy of the human placenta. Anat. Rec. 123,133–149.Google Scholar
  33. Wynn, R.M. (1972) Cytotrophoblastic specializations: An ultrastructual study of the human placenta. Am. J. Obstet. Gynecol. 114, 339–353.Google Scholar
  34. Zeitler, P., Markoff, E. and Handwerger, S. (1983) Characterization of the synthesis and release of human placental lactogen and human chorionic gonadotropin by an enriched population of dispersed placental cells. J. Clin. Endocrinol. Metab. 57, 812–818.Google Scholar

Copyright information

© Springer Science+Business Media New York 1987

Authors and Affiliations

  • Juliet O. Lobo
    • 1
  • Francis L. Bellino
    • 1
  • Alan Siegel
    • 1
  1. 1.Dept. of Biological SciencesState University of New York at BuffaloBuffaloUSA

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